Paris/July 26

From 2007.igem.org

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* 8.5 µl VF2
* 8.5 µl VF2
* 8.5 µl VR
* 8.5 µl VR
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12 µl of the mix in 13µl of lysed cells
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12 µl of the mix in 13µl of lysed cells for each PCR reaction
'''Cycle x30:'''
'''Cycle x30:'''

Revision as of 16:27, 27 July 2007

Colonies PCR for cloning verification

L1.1, L1.2, L2.1, L2.2, L9.1, L10.1

L6: 8 clones

Toothpick in 50µl sterile water ==> 10'@100°C

PCR Mix:

  • 212.5 µl Master Mix
  • 8.5 µl VF2
  • 8.5 µl VR

12 µl of the mix in 13µl of lysed cells for each PCR reaction

Cycle x30:

  • 95°C 30
  • 60°C 30
  • 68°C 2'