Paris/July 8
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Nicolas C. (Talk | contribs) |
Nicolas C. (Talk | contribs) |
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|H5=H<sub>2</sub>0|H6=H<sub>2</sub>0|H7=H<sub>2</sub>0|H8=H<sub>2</sub>0 | |H5=H<sub>2</sub>0|H6=H<sub>2</sub>0|H7=H<sub>2</sub>0|H8=H<sub>2</sub>0 | ||
|H9=H<sub>2</sub>0|H10=H<sub>2</sub>0|H11=H<sub>2</sub>0|H12=H<sub>2</sub>0}} | |H9=H<sub>2</sub>0|H10=H<sub>2</sub>0|H11=H<sub>2</sub>0|H12=H<sub>2</sub>0}} | ||
| + | The 96 plates is surrounded by H<sub>2</sub>O in order to maintain humidity rate during acquisition of the kinetic. In each slot, 200µL of the growth medium (LB or S0.x) is mixed with 2µL of w121 culture grown ON, and with different amount of DAP (see table). | ||
| + | The kinetic is measured during 20H, one point is acquired each 4min10s. | ||
Revision as of 15:24, 11 July 2007
Growth kinetics of w121 strain
We make an array to test growth of w121 on different growth media (LB, S0.2, S0.4, S0.6, S0.8), supplemented with different amount of DAP.
| Kinetic Array :le titre ici | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 |
| B | H20 | LB+0µM DAP | LB+13µM DAP | LB+17µM DAP | LB+20µM DAP | LB+23µM DAP | LB+27µM DAP | LB+30µM DAP | LB+33µM DAP | LB+37µM DAP | LB+40µM DAP | H20 |
| C | H20 | S0.2+0µM DAP | S0.2+13µM DAP | S0.2+17µM DAP | S0.2+20µM DAP | S0.2+23µM DAP | S0.2+27µM DAP | S0.2+30µM DAP | S0.2+33µM DAP | S0.2+37µM DAP | S0.2+40µM DAP | H20 |
| D | H20 | S0.4+0µM DAP | S0.4+13µM DAP | S0.4+17µM DAP | S0.4+20µM DAP | S0.4+23µM DAP | S0.4+27µM DAP | S0.4+30µM DAP | S0.4+33µM DAP | S0.4+37µM DAP | S0.4+40µM DAP | H20 |
| E | H20 | S0.4+0µM DAP | S0.4+13µM DAP | S0.4+17µM DAP | S0.4+20µM DAP | S0.4+23µM DAP | S0.4+27µM DAP | S0.4+30µM DAP | S0.4+33µM DAP | S0.4+37µM DAP | S0.4+40µM DAP | H20 |
| F | H20 | S0.6+0µM DAP | S0.6+13µM DAP | S0.6+17µM DAP | S0.6+20µM DAP | S0.6+23µM DAP | S0.6+27µM DAP | S0.6+30µM DAP | S0.6+33µM DAP | S0.6+37µM DAP | S0.6+40µM DAP | H20 |
| G | H20 | S0.8+0µM DAP | S0.8+13µM DAP | S0.8+17µM DAP | S0.8+20µM DAP | S0.8+23µM DAP | S0.8+27µM DAP | S0.8+30µM DAP | S0.8+33µM DAP | S0.8+37µM DAP | S0.8+40µM DAP | H20 |
| H | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 |
The 96 plates is surrounded by H2O in order to maintain humidity rate during acquisition of the kinetic. In each slot, 200µL of the growth medium (LB or S0.x) is mixed with 2µL of w121 culture grown ON, and with different amount of DAP (see table). The kinetic is measured during 20H, one point is acquired each 4min10s.
