Paris/Modeling char

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* The promoters:
* The promoters:
-
      -DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of            DAPa repression. look the arabinose AraC  
+
-DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of             
-
      -the high constitutive promoter that we are using:<bbpart>BBa_J23112</bbpart>
+
repression. look the arabinose AraC  
 +
 
 +
-the high constitutive promoter that we are using:<bbpart>BBa_J23112</bbpart>
*cre efficience :
*cre efficience :
 +
 +
 +
<bbpart>BBa_J5528</bbpart> :Arabinose -> pBad = GFP : use it to test pbad efficiency,or make a similar construct
 +
 +
assemble Arabinose and cre one one plasmide,  loxP ccdb loxP on another, and test the recombination frequency
 +
 +
 +
[[Paris/Modeling|<<Back to modelling]]

Latest revision as of 15:18, 19 July 2007

We must characterise the different part we are using. Essentially :

  • The promoters:

-DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of repression. look the arabinose AraC

-the high constitutive promoter that we are using:BBa_J23112

  • cre efficience :


BBa_J5528 :Arabinose -> pBad = GFP : use it to test pbad efficiency,or make a similar construct

assemble Arabinose and cre one one plasmide, loxP ccdb loxP on another, and test the recombination frequency


<<Back to modelling