Paris/Modeling char
From 2007.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
* The promoters: | * The promoters: | ||
| - | -DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of | + | #-DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of |
repression. look the arabinose AraC | repression. look the arabinose AraC | ||
| - | -the high constitutive promoter that we are using:<bbpart>BBa_J23112</bbpart> | + | #-the high constitutive promoter that we are using:<bbpart>BBa_J23112</bbpart> |
*cre efficience : | *cre efficience : | ||
Revision as of 13:37, 19 July 2007
We must characterise the different part we are using. Essentially :
- The promoters:
- -DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of
repression. look the arabinose AraC
- -the high constitutive promoter that we are using:BBa_J23112
- cre efficience :
BBa_J5528 :Arabinose -> pBad = GFP : use it to test pbad efficiency,or make a similar construct
assemble Arabinose and cre one one plasmide, loxP ccdb loxP on another, and test the recombination frequency
