Paris/Modeling char

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Latest revision as of 15:18, 19 July 2007

We must characterise the different part we are using. Essentially :

  • The promoters:

-DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of repression. look the arabinose AraC

-the high constitutive promoter that we are using:BBa_J23112

  • cre efficience :


BBa_J5528 :Arabinose -> pBad = GFP : use it to test pbad efficiency,or make a similar construct

assemble Arabinose and cre one one plasmide, loxP ccdb loxP on another, and test the recombination frequency


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