Paris/October 10
From 2007.igem.org
< Paris(Difference between revisions)
(→Preparing competent FX85 cells for Recombination Rate Test) |
|||
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
[[Paris/October 9|yesterday]] -- [[Paris/October 11|tomorrow]] <br> | [[Paris/October 9|yesterday]] -- [[Paris/October 11|tomorrow]] <br> | ||
== PCR on Colony == | == PCR on Colony == | ||
| + | |||
| + | * L52 (O18 & O7) ---> give nothing | ||
| + | * L53 (O18 & O7) ---> give nothing | ||
| + | * L55 (O18 & O9) ---> clone 14 & 16 OK | ||
== Preparing competent FX85 cells for Recombination Rate Test == | == Preparing competent FX85 cells for Recombination Rate Test == | ||
| + | |||
| + | * 1- Classic protocol for preparing TSS chemically competent bacteria<br> | ||
| + | * 2- Classic transformation protocol (1µL of Miniprep), except that we don't use an expression period before starting to put the antibiotics (Kan and Amp)<br> | ||
| + | * 3- We | ||
== Recombination Rate Test == | == Recombination Rate Test == | ||
== Culture of FR781 for FACS == | == Culture of FR781 for FACS == | ||
Latest revision as of 16:21, 11 October 2007
Contents |
PCR on Colony
- L52 (O18 & O7) ---> give nothing
- L53 (O18 & O7) ---> give nothing
- L55 (O18 & O9) ---> clone 14 & 16 OK
Preparing competent FX85 cells for Recombination Rate Test
- 1- Classic protocol for preparing TSS chemically competent bacteria
- 2- Classic transformation protocol (1µL of Miniprep), except that we don't use an expression period before starting to put the antibiotics (Kan and Amp)
- 3- We
