Paris/October 18

From 2007.igem.org

yesterday -- tomorrow

Cultures launched

  • FR781 (from glycerol & plates) in LB-Dap-Kan
  • S50 (FR781 transformed with dapAp>>mrfp) (from glycerol & plates) in LB-Dap-Kan-Amp
  • S16.2 (araC/pBAD>>mrfp in DH5a) in LB-Amp
  • S27.1 (pTet>>mrfp in DH5a) in LB-Amp
  • DH5a in LB


PCR rxn

PCR : PrefixBB-frt1-CmR-frt2-T1T2-SufixBB
PCR Settings Buffer (5x) 5x 10µL (GC rich) Expected size
Annealing (°C) MgCl2 10µM 10µM 0µL (no extra)
60 dNTP 10µM 10µM 1µL Success
Time Elongation Oligo F 10µM O51 10µM 2.5µL NO
2'30 Oligo R 10µM O53 10µM 2.5µL Image (click to enlarge)
Number cycles Water qsp 50µL [[Image:|30px]]
33 Polymerase Phusion 0,5µL Band (0=ladder)
DNA pY-2P-IntC 1µL (dil. 1/10)
PCR : PrefixBB-mutDGAT-SufixBB
PCR Settings Buffer (5x) 5x 10µL (GC rich) Expected size
Annealing (°C) MgCl2 10µM 10µM 0µL (no extra)
60 dNTP 10µM 10µM 1µL Success
Time Elongation Oligo F 10µM O29 10µM 2.5µL NO
2'30 Oligo R 10µM O30 10µM 2.5µL Image (click to enlarge)
Number cycles Water qsp 50µL [[Image:|30px]]
33 Polymerase Phusion 0,5µL Band (0=ladder)
DNA P13 2.5µL