Paris/October 21

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yesterday -- tomorrow

Contents

Miniprep

The 80mL culture have been treated as a 10mL culture and lysate have been put through 2 different columns. We eluted the DNA in 2*100µL. We finally recovered 2µg of DNA in 200µL for a concentration of 10ng/µL.

Transformation of Ligation products

Purification of PCR products

Digestion Reactions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D82 frt1-CmR-frt2 FI P16 EcoRI SpeI frt1-CmR-frt2 PCR product digested for use as a FI
D83 frt1-CmR-frt2 BI P16 XbaI PstI frt1-CmR-frt2 PCR product digested for use as a BI
D84 frt1-CmR-frt2 BBi P16 EcoRI PstI frt1-CmR-frt2 PCR product digested for cloning as a BB
D96 frt1-CmR-frt2-T1T2 FI P16 EcoRI SpeI frt1-CmR-frt2-T1T2 PCR product digested for use as a FI
D97 frt1-CmR-frt2-T1T2 BI P16 XbaI PstI frt1-CmR-frt2-T1T2 PCR product digested for use as a BI
D98 frt1-CmR-frt2-T1T2 BBi P16 EcoRI PstI frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB
D95 PacI[pY-2C-IntC]SmaI pY-2C-IntC plasmid PacI SmaI pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning