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	== Sequencing reactions ==		== Sequencing reactions ==
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-	In order to verify the quality of the ligation reaction products (of [[Paris/September 18| Sep. 18]]), the following sequencing reactions are being performed
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-	TL_L44.1+O18
-	TL_L44.1+O19
-	TL_L44.2+O18
-	TL_L44.2+O19
-	TL_L45.1+O18
-	TL_L45.1+O19
-	TL_L45.2+O18
-	TL_L45.2+O19
-	TL_L46.1+O18
-	TL_L46.1+O19
-	TL_L47.1+O18
-	TL_L47.1+O19
-	TL_L53.7+O18
-	TL_L53.7+O19
-	TL_L53.7+O26
-	TL_L54.3+O18
-	TL_L54.3+O19
-	TL_L54.3+O26
-	TL_L54.4+O18
-	TL_L54.4+O19
-	TL_L54.4+O26
-	TL_L56.3+O18
-	TL_L56.3+O19
-	TL_L56.3+O25
-	TL_L57.3+O18
-	TL_L57.3+O19
-	TL_L57.3+O25
-	TL_L57.4+O18
-	TL_L57.4+O19
-	TL_L57.4+O25
-	TL_L58.5+O18
-	TL_L58.5+O19
-	TL_L58.7+O18
-	TL_L58.7+O19
-	TL_L59.6+O18
-	TL_L59.6+O19
-	TL_L59.6+O40
-	TL_L63.1+O18
-	TL_L63.1+O19
-	TL_L63.2+O18
-	TL_L63.2+O19
-	TL_L64.1+O18
-	TL_L64.1+O19
-	TL_L64.1+O25
-	TL_L64.2+O18
-	TL_L64.2+O19
-	TL_L64.2+O25
	== Transformation of W121 and FR781 didn't work ==		== Transformation of W121 and FR781 didn't work ==

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Revision as of 17:17, 3 October 2007

Contents 1 Sequencing reactions 2 Transformation of W121 and FR781 didn't work 3 Culture for miniprep of S49 having the pKD46 plamid 4 Culture for miniprep of a strain (S51) having the plasmid pY-2P-IntC 5 The wanner PCR didn't give the right size, exploration for explanation

Sequencing reactions

Transformation of W121 and FR781 didn't work

Culture for miniprep of S49 having the pKD46 plamid

Culture for miniprep of a strain (S51) having the plasmid pY-2P-IntC

The wanner PCR didn't give the right size, exploration for explanation