Paris/October 3

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(Sequencing reactions)
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== Sequencing reactions ==
== Sequencing reactions ==
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In order to verify the quality of the ligation reaction products (of [[Paris/September 18| Sep. 18]]), the following sequencing reactions are being performed
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TL_L44.1+O18
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TL_L44.1+O19
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TL_L44.2+O18
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TL_L44.2+O19
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TL_L45.1+O18
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TL_L45.1+O19
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TL_L45.2+O18
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TL_L45.2+O19
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TL_L46.1+O18
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TL_L46.1+O19
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TL_L47.1+O18
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TL_L47.1+O19
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TL_L53.7+O18
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TL_L53.7+O19
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TL_L53.7+O26
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TL_L54.3+O18
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TL_L54.3+O19
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TL_L54.3+O26
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TL_L54.4+O18
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TL_L54.4+O19
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TL_L54.4+O26
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TL_L56.3+O18
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TL_L56.3+O19
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TL_L56.3+O25
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TL_L57.3+O18
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TL_L57.3+O19
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TL_L57.3+O25
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TL_L57.4+O18
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TL_L57.4+O19
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TL_L57.4+O25
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TL_L58.5+O18
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TL_L58.5+O19
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TL_L58.7+O18
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TL_L58.7+O19
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TL_L59.6+O18
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TL_L59.6+O19
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TL_L59.6+O40
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TL_L63.1+O18
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TL_L63.1+O19
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TL_L63.2+O18
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TL_L63.2+O19
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TL_L64.1+O18
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TL_L64.1+O19
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TL_L64.1+O25
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TL_L64.2+O18
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TL_L64.2+O19
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TL_L64.2+O25
== Transformation of W121 and FR781 didn't work ==
== Transformation of W121 and FR781 didn't work ==

Revision as of 17:21, 3 October 2007

Contents

Sequencing reactions

In order to verify the quality of the ligation reaction products (of Sep. 18), the following sequencing reactions are being performed

TL_L44.1+O18 TL_L44.1+O19 TL_L44.2+O18 TL_L44.2+O19 TL_L45.1+O18 TL_L45.1+O19 TL_L45.2+O18 TL_L45.2+O19 TL_L46.1+O18 TL_L46.1+O19 TL_L47.1+O18 TL_L47.1+O19 TL_L53.7+O18 TL_L53.7+O19 TL_L53.7+O26 TL_L54.3+O18 TL_L54.3+O19 TL_L54.3+O26 TL_L54.4+O18 TL_L54.4+O19 TL_L54.4+O26 TL_L56.3+O18 TL_L56.3+O19 TL_L56.3+O25 TL_L57.3+O18 TL_L57.3+O19 TL_L57.3+O25 TL_L57.4+O18 TL_L57.4+O19 TL_L57.4+O25 TL_L58.5+O18 TL_L58.5+O19 TL_L58.7+O18 TL_L58.7+O19 TL_L59.6+O18 TL_L59.6+O19 TL_L59.6+O40 TL_L63.1+O18 TL_L63.1+O19 TL_L63.2+O18 TL_L63.2+O19 TL_L64.1+O18 TL_L64.1+O19 TL_L64.1+O25 TL_L64.2+O18 TL_L64.2+O19 TL_L64.2+O25

Transformation of W121 and FR781 didn't work

Culture for miniprep of S49 having the pKD46 plamid

Culture for miniprep of a strain (S51) having the plasmid pY-2P-IntC

The wanner PCR didn't give the right size, exploration for explanation