From 2007.igem.org

Contents 1 Sequencing reactions 2 Transformation of W121 and FR781 didn't work 3 Culture for miniprep of S49 having the pKD46 plamid 4 Culture for miniprep of a strain (S51) having the plasmid pY-2P-IntC 5 The wanner PCR didn't give the right size, exploration for explanation

Sequencing reactions

In order to verify the quality of the ligation reaction products (of Sep. 18), the following sequencing reactions are being performed

TL_L44.1+O18 TL_L44.1+O19 TL_L44.2+O18 TL_L44.2+O19 TL_L45.1+O18 TL_L45.1+O19 TL_L45.2+O18 TL_L45.2+O19 TL_L46.1+O18 TL_L46.1+O19 TL_L47.1+O18 TL_L47.1+O19 TL_L53.7+O18 TL_L53.7+O19 TL_L53.7+O26 TL_L54.3+O18 TL_L54.3+O19 TL_L54.3+O26 TL_L54.4+O18 TL_L54.4+O19 TL_L54.4+O26 TL_L56.3+O18 TL_L56.3+O19 TL_L56.3+O25 TL_L57.3+O18 TL_L57.3+O19 TL_L57.3+O25 TL_L57.4+O18 TL_L57.4+O19 TL_L57.4+O25 TL_L58.5+O18 TL_L58.5+O19 TL_L58.7+O18 TL_L58.7+O19 TL_L59.6+O18 TL_L59.6+O19 TL_L59.6+O40 TL_L63.1+O18 TL_L63.1+O19 TL_L63.2+O18 TL_L63.2+O19 TL_L64.1+O18 TL_L64.1+O19 TL_L64.1+O25 TL_L64.2+O18 TL_L64.2+O19 TL_L64.2+O25

Transformation of W121 and FR781 didn't work

Culture for miniprep of S49 having the pKD46 plamid

Culture for miniprep of a strain (S51) having the plasmid pY-2P-IntC

The wanner PCR didn't give the right size, exploration for explanation