Paris/October 8

From 2007.igem.org

(Difference between revisions)
(Digestion products)
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== Digestion products ==
== Digestion products ==
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{|
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|- style="background: #ccccff;"
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! colspan="5" style="background: #ccccff;" | Digestion Products
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|- style="background: #ccccff; text-align: center;"
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|width=5%| Number
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|width=24%| Product Name
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|width=24%| Matrix Name
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|width=5%| Enzyme 1
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|width=5%| Enzyme 2
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|width=5%| Size
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|width=34%| Description
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|width=1%|
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|width=1%|
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|width=1%|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D79
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|
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|L44.1
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|Xba1
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|Pst1
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|
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|
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|
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D80
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|
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|L44.2
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|Xba1
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|Pst1
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|
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|
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|
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D81
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|
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|L45.1
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|Xba1
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|Pst1
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|
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|
 +
|
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D82
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|
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|L45.2
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|Xba1
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|Pst1
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|
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|
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|
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D83
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|
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|L46.1
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|Xba1
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|Pst1
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|
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|
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|
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D84
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|
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|L47.1
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|Xba1
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|Pst1
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|
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|
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|
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D85
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|
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|L53.7
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|EcoRI
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|SpeI
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|
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|
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|
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D86
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|
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|L54.3
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|EcoRI
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|SpeI
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|
 +
|
 +
|
 +
|
 +
|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D87
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|
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|L54.4
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|EcoRI
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|SpeI
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|
 +
|
 +
|
 +
|
 +
|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D88
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|
 +
|L56.3
 +
|EcoRI
 +
|SpeI
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|
 +
|
 +
|
 +
|
 +
|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D89
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|
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|L57.1
 +
|EcoRI
 +
|SpeI
 +
|
 +
|
 +
|
 +
|
 +
|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D90
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|
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|L57.3
 +
|EcoRI
 +
|SpeI
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|
 +
|
 +
|
 +
|
 +
|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D91
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|
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|MP14.1
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|EcoRI
 +
|XbaI
 +
|
 +
|
 +
|
 +
|
 +
|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D92
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|
 +
|MP14.2
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|EcoRI
 +
|XbaI
 +
|
 +
|
 +
|
 +
|
 +
|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D93
 +
|
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|MP14.1
 +
|Spe1
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|Pst1
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|
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|
 +
|
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D94
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|
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|MP14.2
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|Spe1
 +
|Pst1
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|
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|
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|
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|
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|
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|}
== Ligation ==
== Ligation ==

Revision as of 17:02, 9 October 2007

Contents

Digestion products

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D79 L44.1 Xba1 Pst1
D80 L44.2 Xba1 Pst1
D81 L45.1 Xba1 Pst1
D82 L45.2 Xba1 Pst1
D83 L46.1 Xba1 Pst1
D84 L47.1 Xba1 Pst1
D85 L53.7 EcoRI SpeI
D86 L54.3 EcoRI SpeI
D87 L54.4 EcoRI SpeI
D88 L56.3 EcoRI SpeI
D89 L57.1 EcoRI SpeI
D90 L57.3 EcoRI SpeI
D91 MP14.1 EcoRI XbaI
D92 MP14.2 EcoRI XbaI
D93 MP14.1 Spe1 Pst1
D94 MP14.2 Spe1 Pst1

Ligation

Transformation

Cultures of FR781 for pDapA caracterisation by FACS

Culture of FX85 for future competent transformation and then recombination rate caracterisation