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Revision as of 13:58, 11 October 2007 (view source) Landrain (Talk | contribs) (→Digestion products) ← Older edit		Revision as of 14:04, 11 October 2007 (view source) Landrain (Talk | contribs) (→Digestion products) Newer edit →
Line 18:		Line 18:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D79		| style="background: #ccffcc;" |D79
-	|	+	|pJ23100>>lox71-B0015(T)
	|L44.1		|L44.1
	|Xba1		|Xba1
	|Pst1		|Pst1
	|		|
-	|	+	|BI
	|		|
	|		|
Line 29:		Line 29:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D80		| style="background: #ccffcc;" |D80
-	|	+	|pJ23100>>lox71-B0015(T)
	|L44.2		|L44.2
	|Xba1		|Xba1
	|Pst1		|Pst1
	|		|
-	|	+	|BI
	|		|
	|		|
Line 40:		Line 40:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D81		| style="background: #ccffcc;" |D81
-	|	+	|pJ23107>>lox71-B0015(T)
	|L45.1		|L45.1
	|Xba1		|Xba1
	|Pst1		|Pst1
	|		|
-	|	+	|BI
	|		|
	|		|
Line 51:		Line 51:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D82		| style="background: #ccffcc;" |D82
-	|	+	|pJ23107>>lox71-B0015(T)
	|L45.2		|L45.2
	|Xba1		|Xba1
	|Pst1		|Pst1
	|		|
-	|	+	|BI
	|		|
	|		|
Line 62:		Line 62:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D83		| style="background: #ccffcc;" |D83
-	|	+	|pTet>>lox71-B0015(T)
	|L46.1		|L46.1
	|Xba1		|Xba1
	|Pst1		|Pst1
	|		|
-	|	+	|BI
	|		|
	|		|
Line 73:		Line 73:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D84		| style="background: #ccffcc;" |D84
-	|	+	|araC/pBad>>lox71-B0015(T)
	|L47.1		|L47.1
	|Xba1		|Xba1
	|Pst1		|Pst1
	|		|
-	|	+	|BI
	|		|
	|		|
Line 84:		Line 84:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D85		| style="background: #ccffcc;" |D85
-	|	+	|B0015(T)-lox66-RBSDapAcoli
	|L53.7		|L53.7
	|EcoRI		|EcoRI
	|SpeI		|SpeI
	|		|
-	|	+	|FI
	|		|
	|		|
Line 95:		Line 95:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D86		| style="background: #ccffcc;" |D86
-	|	+	|attB-lox66-RBSDapAcoli
	|L54.3		|L54.3
	|EcoRI		|EcoRI
	|SpeI		|SpeI
	|		|
-	|	+	|FI
	|		|
	|		|
Line 106:		Line 106:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D87		| style="background: #ccffcc;" |D87
-	|	+	|attB-lox66-RBSDapAcoli
	|L54.4		|L54.4
	|EcoRI		|EcoRI
	|SpeI		|SpeI
	|		|
-	|	+	|FI
	|		|
	|		|
Line 117:		Line 117:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D88		| style="background: #ccffcc;" |D88
-	|	+	|B0015(T)-lox66-RBSdapAsubtilis
	|L56.3		|L56.3
	|EcoRI		|EcoRI
	|SpeI		|SpeI
	|		|
-	|	+	|FI
	|		|
	|		|
Line 128:		Line 128:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D89		| style="background: #ccffcc;" |D89
-	|	+	|attB-lox66-RBSdapAsubtilis
	|L57.1		|L57.1
	|EcoRI		|EcoRI
	|SpeI		|SpeI
	|		|
-	|	+	|FI
	|		|
	|		|
Line 139:		Line 139:
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |D90		| style="background: #ccffcc;" |D90
-	|	+	|attB-lox66-RBSdapAsubtilis
	|L57.3		|L57.3
	|EcoRI		|EcoRI
	|SpeI		|SpeI
	|		|
-	|	+	|FI
-	|	+
-	|	+
-	|	+
-	|- style="background: #cccccc;"	+
-	| style="background: #ccffcc;" |D91	+
-	|<bbpart>BBa_J61025</bbpart>	+
-	|MP14.1	+
-	|EcoRI	+
-	|XbaI	+
-	|	+
-	|Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>BBa_J61025 SpecifiedComponents</partinfo>	+
-	|	+
-	|	+
-	|	+
-	|- style="background: #cccccc;"	+
-	| style="background: #ccffcc;" |D92	+
-	|<bbpart>BBa_J61025</bbpart>	+
-	|MP14.2	+
-	|EcoRI	+
-	|XbaI	+
-	|	+
-	|Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>BBa_J61025 SpecifiedComponents</partinfo>	+
-	|	+
-	|	+
-	|	+
-	|- style="background: #cccccc;"	+
-	| style="background: #ccffcc;" |D93	+
-	|<bbpart>BBa_J61025</bbpart>	+
-	|MP14.1	+
-	|Spe1	+
-	|Pst1	+
-	|	+
-	|Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>BBa_J61025 SpecifiedComponents</partinfo>	+
-	|	+
-	|	+
-	|	+
-	|- style="background: #cccccc;"	+
-	| style="background: #ccffcc;" |D94	+
-	|<bbpart>BBa_J61025</bbpart>	+
-	|MP14.2	+
-	|Spe1	+
-	|Pst1	+
-	|	+
-	|Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>BBa_J61025 SpecifiedComponents</partinfo>	+
	|		|
	|		|

---

Revision as of 14:04, 11 October 2007

yesterday -- tomorrow

Contents 1 Digestion products 2 Ligation 3 Transformation 4 Cultures of FR781 for pDapA caracterisation by FACS 5 Culture of FX85 for future competent transformation and then recombination rate caracterisation

Digestion products

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Size	Description
D79	pJ23100>>lox71-B0015(T)	L44.1	Xba1	Pst1		BI
D80	pJ23100>>lox71-B0015(T)	L44.2	Xba1	Pst1		BI
D81	pJ23107>>lox71-B0015(T)	L45.1	Xba1	Pst1		BI
D82	pJ23107>>lox71-B0015(T)	L45.2	Xba1	Pst1		BI
D83	pTet>>lox71-B0015(T)	L46.1	Xba1	Pst1		BI
D84	araC/pBad>>lox71-B0015(T)	L47.1	Xba1	Pst1		BI
D85	B0015(T)-lox66-RBSDapAcoli	L53.7	EcoRI	SpeI		FI
D86	attB-lox66-RBSDapAcoli	L54.3	EcoRI	SpeI		FI
D87	attB-lox66-RBSDapAcoli	L54.4	EcoRI	SpeI		FI
D88	B0015(T)-lox66-RBSdapAsubtilis	L56.3	EcoRI	SpeI		FI
D89	attB-lox66-RBSdapAsubtilis	L57.1	EcoRI	SpeI		FI
D90	attB-lox66-RBSdapAsubtilis	L57.3	EcoRI	SpeI		FI

Ligation

Transformation

Cultures of FR781 for pDapA caracterisation by FACS

Culture of FX85 for future competent transformation and then recombination rate caracterisation