Paris/October 8
From 2007.igem.org
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== Ligation == | == Ligation == | ||
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| + | I should have perform ligations between those digestions upthere with the digered part [Frt-CmR-Frt] so as to be able to do Wanner later, but taking into consideration that this part had never beared any Cm resistance gene, ligations have to be posponed till this same part be ready to use. | ||
== Transformation == | == Transformation == | ||
Revision as of 14:10, 11 October 2007
Contents |
Digestion products
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D79 | pJ23100>>lox71-B0015(T) | L44.1 | Xba1 | Pst1 | BI | ||||
| D80 | pJ23100>>lox71-B0015(T) | L44.2 | Xba1 | Pst1 | BI | ||||
| D81 | pJ23107>>lox71-B0015(T) | L45.1 | Xba1 | Pst1 | BI | ||||
| D82 | pJ23107>>lox71-B0015(T) | L45.2 | Xba1 | Pst1 | BI | ||||
| D83 | pTet>>lox71-B0015(T) | L46.1 | Xba1 | Pst1 | BI | ||||
| D84 | araC/pBad>>lox71-B0015(T) | L47.1 | Xba1 | Pst1 | BI | ||||
| D85 | B0015(T)-lox66-RBSDapAcoli | L53.7 | EcoRI | SpeI | FI | ||||
| D86 | attB-lox66-RBSDapAcoli | L54.3 | EcoRI | SpeI | FI | ||||
| D87 | attB-lox66-RBSDapAcoli | L54.4 | EcoRI | SpeI | FI | ||||
| D88 | B0015(T)-lox66-RBSdapAsubtilis | L56.3 | EcoRI | SpeI | FI | ||||
| D89 | attB-lox66-RBSdapAsubtilis | L57.1 | EcoRI | SpeI | FI | ||||
| D90 | attB-lox66-RBSdapAsubtilis | L57.3 | EcoRI | SpeI | FI | ||||
Ligation
I should have perform ligations between those digestions upthere with the digered part [Frt-CmR-Frt] so as to be able to do Wanner later, but taking into consideration that this part had never beared any Cm resistance gene, ligations have to be posponed till this same part be ready to use.
