From 2007.igem.org
yesterday -- tomorrow
Digestion products
Digestion Products
|
Number
| Product Name
| Matrix Name
| Enzyme 1
| Enzyme 2
| Size
| Description
|
|
|
|
D79
| pJ23100>>lox71-B0015(T)
| L44.1
| Xba1
| Pst1
|
| BI
|
|
|
|
D80
| pJ23100>>lox71-B0015(T)
| L44.2
| Xba1
| Pst1
|
| BI
|
|
|
|
D81
| pJ23107>>lox71-B0015(T)
| L45.1
| Xba1
| Pst1
|
| BI
|
|
|
|
D82
| pJ23107>>lox71-B0015(T)
| L45.2
| Xba1
| Pst1
|
| BI
|
|
|
|
D83
| pTet>>lox71-B0015(T)
| L46.1
| Xba1
| Pst1
|
| BI
|
|
|
|
D84
| araC/pBad>>lox71-B0015(T)
| L47.1
| Xba1
| Pst1
|
| BI
|
|
|
|
D85
| B0015(T)-lox66-RBSDapAcoli
| L53.7
| EcoRI
| SpeI
|
| FI
|
|
|
|
D86
| attB-lox66-RBSDapAcoli
| L54.3
| EcoRI
| SpeI
|
| FI
|
|
|
|
D87
| attB-lox66-RBSDapAcoli
| L54.4
| EcoRI
| SpeI
|
| FI
|
|
|
|
D88
| B0015(T)-lox66-RBSdapAsubtilis
| L56.3
| EcoRI
| SpeI
|
| FI
|
|
|
|
D89
| attB-lox66-RBSdapAsubtilis
| L57.1
| EcoRI
| SpeI
|
| FI
|
|
|
|
D90
| attB-lox66-RBSdapAsubtilis
| L57.3
| EcoRI
| SpeI
|
| FI
|
|
|
|
Ligation
I should have perform ligations between those digestions upthere with the digered part [Frt-CmR-Frt] so as to be able to do Wanner later, but taking into consideration that this part had never beared any Cm resistance gene, ligations have to be posponed till this same part be ready to use.
Transformation
Cultures of FR781 for pDapA caracterisation by FACS
Culture of FX85 for future competent transformation and then recombination rate caracterisation