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| | [[Paris/September 12|yesterday]] -- [[Paris/September 14|tomorrow]] | | [[Paris/September 12|yesterday]] -- [[Paris/September 14|tomorrow]] |
| - |
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| - | == Digestions ==
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| - |
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| - | {|
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| - | |- style="background: #ccccff;"
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| - | ! colspan="5" style="background: #ccccff;" | Digestion Products
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| - | |- style="background: #ccccff; text-align: center;"
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| - | |width=5%| Number
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| - | |width=24%| Product Name
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| - | |width=24%| Matrix Name
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| - | |width=5%| Enzyme 1
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| - | |width=5%| Enzyme 2
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| - | |width=5%| Size
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| - | |width=34%| Description
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| - | |width=1%|
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| - | |width=1%|
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| - | |width=1%|
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| - | |- style="background: #cccccc;"
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| - | | style="background: #FF99FF;" |D56
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| - | |araC/pBad>>RBS-Cre BBinsert
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| - | |L26.1 araC/pbad>>RBS-Cre in <bbpart>J61002</bbpart>
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| - | |EcoRI
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| - | |Pst1
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| - | |
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| - | |araC/pBad>>RBS-Cre BBinsert (for transfer to the low-copy plasmid vector)
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D57
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| - | |lox71-B0015(T) BI
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| - | |L31.1, L31.3, L31.4 lox71-B0015(T) <bbpart>pSB1A2</bbpart>
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| - | |XbaI
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| - | |Pst1
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| - | |
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| - | |lox71-B0015(T) extracted for use as a BI <bbpart>pSB1A2</bbpart>
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #FF99FF;" |D58
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| - | |B0015(T)-lox66 BV
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| - | |L32.1, L32.2, L32.3 B0015(T)-lox66 BV
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| - | |SpeI
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| - | |PstI
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| - | |
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| - | |B0015(T)-lox66 for use as a BV <bbpart>pSB1A2</bbpart>
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D59
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| - | |pTet>>lox71-gfptripart BI
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| - | |L34.1, L34.2, L34.3 pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
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| - | |XbaI
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| - | |PstI
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| - | |
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| - | |pTet>>lox71-gfptripart for use as a BI
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D60
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| - | |araC/pBad>>lox71-gfptripart BI
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| - | |L35.1, L35.2 & L35.3 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
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| - | |XbaI
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| - | |PstI
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| - | |
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| - | |araC/pBad>>lox71-gfptripart for use as a BI
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #FF99FF;" |D61
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| - | |lox66-mRFP FV
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| - | |L36.1, L36.2 & L36.3 lox66-mRFP
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| - | |EcoRI
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| - | |XbaI
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| - | |
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| - | |lox66-gfptri part (strongRBS-ORF-T) ready for use as a forward vector
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D62
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| - | |lox66-RBSDapAcoli BI
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| - | |L37.1, L37.2 & L37.3 lox66-RBSDapAcoli
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| - | |XbaI
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| - | |PstI
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| - | |
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| - | |lox66-RBSDapAcoli ready for use as a Backward insert
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D63
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| - | |RBSDapAcoli BI
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| - | |L39.1, L39.2 & L39.3 RBSDapAcoli
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| - | |XbaI
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| - | |PstI
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| - | |
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| - | |RBSDapAcoli BI (for insertion behind T-lox66)
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #FF99FF;" |D64
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| - | |FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> BV
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| - | |MP14.1 <bbpart>BBa_J61025</bbpart>
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| - | |SpeI
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| - | |PstI
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| - | |
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| - | |FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for backward introduction of a casette for Wanner K insertion)
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #FF99FF;" |D65
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| - | |FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> FV
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| - | |MP14.1 <bbpart>BBa_J61025</bbpart>
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| - | |EcoR
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| - | |XbaI
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| - | |
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| - | |FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for forward introduction of a casette for Wanner K insertion)
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #FF99FF;" |D65
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| - | |KanR <bbpart>BBa_J61025</bbpart> FV
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| - | |MP12.1 <bbpart>BBa_P1003</bbpart> in <bbpart>pSB1A2</bbpart>
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| - | |EcoR
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| - | |XbaI
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| - | |
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| - | |KanR <bbpart>BBa_J61025</bbpart> (for forward introduction of a casette for Wanner K insertion)
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D66
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| - | |RBS-DapAsubtilis BI
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| - | |L43.1, L43.2 & L43.3 RBS-DapAsubtilis <bbpart>pSB1A2</bbpart>
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| - | |XbaI
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| - | |PstI
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| - | |
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| - | |RBS-DapAsubtilis for use as a BI
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #FF99FF;" |D67
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| - | |attB BV
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| - | |MP15.1
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| - | |SpeI
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| - | |PstI
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| - | |
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| - | |vector containing attB for use as a BV
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #FF99FF;" |D68
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| - | |gfptripart-lox66 BV
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| - | |L29.1 gfptripart-lox66 <bbpart>pSB1A2</bbpart>
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| - | |SpeI
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| - | |PstI
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| - | |
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| - | |
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| - | |
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| - | |
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| - | |
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| - | |}
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| - |
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| - | digestion mix:
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| - | * 25µl plasmid
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| - | * 1µl BSA
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| - | * 10 µl NEB2
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| - | * 60µl H2O
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| - | * 2µl Enzyme1
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| - | * 2µl Enzyme2
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