Paris/September 13

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Revision as of 19:44, 13 September 2007 by Eismoustique (Talk | contribs)

yesterday -- tomorrow

Digestions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D56 araC/pBad>>RBS-Cre BBinsert L26.1 araC/pbad>>RBS-Cre in J61002 EcoRI Pst1 araC/pBad>>RBS-Cre BBinsert (for transfer to the low-copy plasmid vector)
D57 lox71-B0015(T) BI L31.1, L31.3, L31.4 lox71-B0015(T) pSB1A2 XbaI Pst1 lox71-B0015(T) extracted for use as a BI pSB1A2
D58 B0015(T)-lox66 BV L32.1, L32.2, L32.3 B0015(T)-lox66 BV SpeI PstI B0015(T)-lox66 for use as a BV pSB1A2
D59 pTet>>lox71-gfptripart BI L34.1, L34.2, L34.3 pTet>>lox71-gfptripart BBa_J61002 XbaI PstI pTet>>lox71-gfptripart for use as a BI
D60 araC/pBad>>lox71-gfptripart BI L35.1, L35.2 & L35.3 araC/pBad>>lox71-gfptripart BBa_J61002 XbaI PstI araC/pBad>>lox71-gfptripart for use as a BI
D61 lox66-mRFP FV L36.1, L36.2 & L36.3 lox66-mRFP EcoRI XbaI lox66-gfptri part (strongRBS-ORF-T) ready for use as a forward vector
D62 lox66-RBSDapAcoli BI L37.1, L37.2 & L37.3 lox66-RBSDapAcoli XbaI PstI lox66-RBSDapAcoli ready for use as a Backward insert
D63 RBSDapAcoli BI L39.1, L39.2 & L39.3 RBSDapAcoli XbaI PstI RBSDapAcoli BI (for insertion behind T-lox66)
D64 FRT-CmR-FRT BBa_J61025 BV MP14.1 BBa_J61025 SpeI PstI FRT-CmR-FRT BBa_J61025 (for backward introduction of a casette for Wanner K insertion)
D65 FRT-CmR-FRT BBa_J61025 FV MP14.1 BBa_J61025 EcoR XbaI FRT-CmR-FRT BBa_J61025 (for forward introduction of a casette for Wanner K insertion)
D65 KanR BBa_J61025 FV MP12.1 BBa_P1003 in pSB1A2 EcoR XbaI KanR BBa_J61025 (for forward introduction of a casette for Wanner K insertion)
D66 RBS-DapAsubtilis BI L43.1, L43.2 & L43.3 RBS-DapAsubtilis pSB1A2 XbaI PstI RBS-DapAsubtilis for use as a BI
D67 attB BV MP15.1 SpeI PstI vector containing attB for use as a BV
D68 gfptripart-lox66 BV L29.1 gfptripart-lox66 pSB1A2 SpeI PstI

digestion mix:

  • 25µl plasmid
  • 1µl BSA
  • 10 µl NEB2
  • 60µl H2O
  • 2µl Enzyme1
  • 2µl Enzyme2