Paris/September 14

From 2007.igem.org

(Difference between revisions)
(→Digestions)
Line 29: Line 29:
| style="background: #ccffcc;" |D57
| style="background: #ccffcc;" |D57
|lox71-B0015(T) BI
|lox71-B0015(T) BI
-
|L31.1, L31.3, L31.4 lox71-B0015(T) <bbpart>pSB1A2</bbpart>
+
|(L31.1, L31.3) lox71-B0015(T) <bbpart>pSB1A2</bbpart>
|XbaI
|XbaI
|Pst1
|Pst1
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| style="background: #FF99FF;" |D58
| style="background: #FF99FF;" |D58
|B0015(T)-lox66 BV
|B0015(T)-lox66 BV
-
|L32.1, L32.2, L32.3 B0015(T)-lox66 BV
+
|(L32.1, L32.2) B0015(T)-lox66 BV
|SpeI
|SpeI
|PstI
|PstI
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| style="background: #ccffcc;" |D59
| style="background: #ccffcc;" |D59
|pTet>>lox71-gfptripart BI
|pTet>>lox71-gfptripart BI
-
|L34.1, L34.2, L34.3 pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
+
|(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
|XbaI
|XbaI
|PstI
|PstI
Line 62: Line 62:
| style="background: #ccffcc;" |D60
| style="background: #ccffcc;" |D60
|araC/pBad>>lox71-gfptripart BI
|araC/pBad>>lox71-gfptripart BI
-
|L35.1, L35.2 & L35.3 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
+
|(L35.1, L35.2) araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
|XbaI
|XbaI
|PstI
|PstI
Line 73: Line 73:
| style="background: #FF99FF;"  |D61
| style="background: #FF99FF;"  |D61
|lox66-mRFP FV
|lox66-mRFP FV
-
|L36.1, L36.2 & L36.3 lox66-mRFP
+
|(L36.1, L36.2) lox66-mRFP
|EcoRI
|EcoRI
|XbaI
|XbaI
|
|
-
|lox66-gfptri part (strongRBS-ORF-T) ready for use as a forward vector
+
|lox66-mRFP part (strongRBS-ORF-T) ready for use as a forward vector
|
|
|
|
Line 84: Line 84:
| style="background: #ccffcc;" |D62
| style="background: #ccffcc;" |D62
|lox66-RBSDapAcoli BI
|lox66-RBSDapAcoli BI
-
|L37.1, L37.2 & L37.3 lox66-RBSDapAcoli  
+
|(L37.1, L37.2) lox66-RBSDapAcoli  
|XbaI
|XbaI
|PstI
|PstI
Line 95: Line 95:
| style="background: #ccffcc;" |D63
| style="background: #ccffcc;" |D63
|RBSDapAcoli BI
|RBSDapAcoli BI
-
|L39.1, L39.2 & L39.3 RBSDapAcoli  
+
|(L39.1, L39.2) RBSDapAcoli  
|XbaI
|XbaI
|PstI
|PstI
Line 106: Line 106:
| style="background: #FF99FF;" |D64
| style="background: #FF99FF;" |D64
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> BV
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> BV
-
|MP14.1 <bbpart>BBa_J61025</bbpart>
+
|(MP14.1) <bbpart>BBa_J61025</bbpart>
|SpeI
|SpeI
|PstI
|PstI
Line 117: Line 117:
| style="background: #FF99FF;" |D65
| style="background: #FF99FF;" |D65
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> FV
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> FV
-
|MP14.1 <bbpart>BBa_J61025</bbpart>
+
|(MP14.1) <bbpart>BBa_J61025</bbpart>
|EcoR
|EcoR
|XbaI
|XbaI
Line 126: Line 126:
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #FF99FF;" |D65
+
| style="background: #FF99FF;" |D66
|KanR <bbpart>BBa_J61025</bbpart> FV
|KanR <bbpart>BBa_J61025</bbpart> FV
-
|MP12.1 <bbpart>BBa_P1003</bbpart> in <bbpart>pSB1A2</bbpart>
+
|(MP12.1) <bbpart>BBa_P1003</bbpart> in <bbpart>pSB1A2</bbpart>
|EcoR
|EcoR
|XbaI
|XbaI
Line 137: Line 137:
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |D66
+
| style="background: #ccffcc;" |D67
|RBS-DapAsubtilis BI
|RBS-DapAsubtilis BI
-
|L43.1, L43.2 & L43.3 RBS-DapAsubtilis <bbpart>pSB1A2</bbpart>
+
|(L43.1, L43.2) RBS-DapAsubtilis <bbpart>pSB1A2</bbpart>
|XbaI
|XbaI
|PstI
|PstI
Line 148: Line 148:
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #FF99FF;" |D67
+
| style="background: #FF99FF;" |D68
|attB BV
|attB BV
-
|MP15.1
+
|(MP15.1)
|SpeI
|SpeI
|PstI
|PstI
Line 159: Line 159:
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #FF99FF;" |D68
+
| style="background: #FF99FF;" |D69
|gfptripart-lox66 BV
|gfptripart-lox66 BV
-
|L29.1 gfptripart-lox66 <bbpart>pSB1A2</bbpart>
+
|(L29.1) gfptripart-lox66 <bbpart>pSB1A2</bbpart>
|SpeI
|SpeI
|PstI
|PstI
|
|
|
|
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D70
 +
|pTet>>lox71-gfptripart FI
 +
|(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
 +
|EcoRI
 +
|SpeI
 +
|
 +
|pTet>>lox71-gfptripart for use as a FI
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D71
 +
|araC/pBad>>lox71-gfptripart FI
 +
|(L35.1, L35.2) araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
 +
|EcoRI
 +
|SpeI
 +
|
 +
|araC/pBad>>lox71-gfptripart for use as a FI
|
|
|
|

Revision as of 15:33, 14 September 2007

Digestions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D56 araC/pBad>>RBS-Cre BBinsert L26.1 araC/pbad>>RBS-Cre in J61002 EcoRI Pst1 araC/pBad>>RBS-Cre BBinsert (for transfer to the low-copy plasmid vector)
D57 lox71-B0015(T) BI (L31.1, L31.3) lox71-B0015(T) pSB1A2 XbaI Pst1 lox71-B0015(T) extracted for use as a BI pSB1A2
D58 B0015(T)-lox66 BV (L32.1, L32.2) B0015(T)-lox66 BV SpeI PstI B0015(T)-lox66 for use as a BV pSB1A2
D59 pTet>>lox71-gfptripart BI (L34.1, L34.2) pTet>>lox71-gfptripart BBa_J61002 XbaI PstI pTet>>lox71-gfptripart for use as a BI
D60 araC/pBad>>lox71-gfptripart BI (L35.1, L35.2) araC/pBad>>lox71-gfptripart BBa_J61002 XbaI PstI araC/pBad>>lox71-gfptripart for use as a BI
D61 lox66-mRFP FV (L36.1, L36.2) lox66-mRFP EcoRI XbaI lox66-mRFP part (strongRBS-ORF-T) ready for use as a forward vector
D62 lox66-RBSDapAcoli BI (L37.1, L37.2) lox66-RBSDapAcoli XbaI PstI lox66-RBSDapAcoli ready for use as a Backward insert
D63 RBSDapAcoli BI (L39.1, L39.2) RBSDapAcoli XbaI PstI RBSDapAcoli BI (for insertion behind T-lox66)
D64 FRT-CmR-FRT BBa_J61025 BV (MP14.1) BBa_J61025 SpeI PstI FRT-CmR-FRT BBa_J61025 (for backward introduction of a casette for Wanner K insertion)
D65 FRT-CmR-FRT BBa_J61025 FV (MP14.1) BBa_J61025 EcoR XbaI FRT-CmR-FRT BBa_J61025 (for forward introduction of a casette for Wanner K insertion)
D66 KanR BBa_J61025 FV (MP12.1) BBa_P1003 in pSB1A2 EcoR XbaI KanR BBa_J61025 (for forward introduction of a casette for Wanner K insertion)
D67 RBS-DapAsubtilis BI (L43.1, L43.2) RBS-DapAsubtilis pSB1A2 XbaI PstI RBS-DapAsubtilis for use as a BI
D68 attB BV (MP15.1) SpeI PstI vector containing attB for use as a BV
D69 gfptripart-lox66 BV (L29.1) gfptripart-lox66 pSB1A2 SpeI PstI
D70 pTet>>lox71-gfptripart FI (L34.1, L34.2) pTet>>lox71-gfptripart BBa_J61002 EcoRI SpeI pTet>>lox71-gfptripart for use as a FI
D71 araC/pBad>>lox71-gfptripart FI (L35.1, L35.2) araC/pBad>>lox71-gfptripart BBa_J61002 EcoRI SpeI araC/pBad>>lox71-gfptripart for use as a FI

digestion mix:

  • 25µl plasmid
  • 1µl BSA
  • 10 µl NEB2
  • 60µl H2O
  • 2µl Enzyme1
  • 2µl Enzyme2
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