http://2007.igem.org/wiki/index.php?title=Paris/September_20&feed=atom&action=historyParis/September 20 - Revision history2024-03-28T13:47:14ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=Paris/September_20&diff=23760&oldid=prevNicolas C. at 18:06, 7 October 20072007-10-07T18:06:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== DapA Wanner deletion: pKD13-DapA homologies PCR ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== DapA Wanner deletion: pKD13-DapA homologies PCR ==</div></td></tr>
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</table>Nicolas C.http://2007.igem.org/wiki/index.php?title=Paris/September_20&diff=19813&oldid=prevDavidpz at 13:22, 20 September 20072007-09-20T13:22:12Z<p></p>
<p><b>New page</b></p><div>== DapA Wanner deletion: pKD13-DapA homologies PCR ==<br />
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The recombination have not worked with the overnight culture on the bench (see yesterday).<br><br />
The new electroporation with the new cells has not worked . <br><br />
The positive control of electroporation (pUC19) shows a few colonies (10) leading to the conclusion of electroporation problem. A new protocol of electrocompetent cells is needed.</div>Davidpz