Paris/September 21
From 2007.igem.org
< Paris
Revision as of 15:02, 21 September 2007 by David.bikard (Talk | contribs)
Electro-competent cells for wanner deletion of DapA
- 100ml culture of MG1655 pKD46 from ON cult.
- grow until DO=0.2
- add arabinose to a final concentration of 0.4%
- grow until DO=0.6
- keep on ice for 10min
- centrifuge 10min at 4000rpm and 4°C
- resuspend in 50ml cold H2O
- centrifuge 10min at 4000rpm and 4°C
- resuspend in 50ml cold H2O
- centrifuge 10min at 4000rpm and 4°C
- resuspend in 25ml cold 10% glycerol
- centrifuge 10min at 4000rpm and 4°C
- resuspend in 200µl cold 10% glycerol
- keep on ice until transformation
