Paris/September 27

From 2007.igem.org

< Paris(Difference between revisions)
(Digestions)
 
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[[Paris/September 26|yesterday]] -- [[Paris/September 28|tomorrow]] <br>
== Digestions ==
== Digestions ==

Latest revision as of 18:07, 7 October 2007

yesterday -- tomorrow

Digestions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D56 araC/pBad>>RBS-Cre BBinsert L26.2 araC/pbad>>RBS-Cre in J61002 EcoRI Pst1 araC/pBad>>RBS-Cre BBinsert (for transfer to the low-copy plasmid vector)
D66 KanR BBa_J61025 FV (MP12.1, MP12.2) BBa_P1003 in pSB1A2 EcoR XbaI KanR BBa_J61025 (for forward introduction of a casette for Wanner K insertion)
D72 pSB2K3 BBvector MP16 pSB2K3::attP EcoRI PstI Low copy number plasmid, BB vector
D73 pSB2K3 ES open vector MP16 pSB2K3::attP EcoRI SpeI Comparison of digestion efficiency between Pst and Spe in NEB2
D75 AraC-pBAD-RBS-CRE FI L26.2 AraC-pBAD-RBS-CRE EcoRI SpeI FI, Comparison of digestion efficiency between Pst and Spe in NEB2 (see D56)