PennState/Project/Diauxie/Setup

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'''Initial Setup'''
'''Initial Setup'''
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To test each of the four xyl promoters, each is placed in a construct followed by a ribosome binding site, the reporter green florescent protein, and a terminator. The strength of each is measured by gfp emission.
 
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<html><img src="http://openwetware.org/images/2/2a/Fptest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylF facing promoter that includes the CRP binding region.
 
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<html><img src="http://openwetware.org/images/d/da/Aptest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylA facing promoter that includes the CRP binding region.
 
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<html><img src="http://openwetware.org/images/b/b1/Fpnctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylA facing promoter that does not include the CRP binding region.
 
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<html><img src="http://openwetware.org/images/d/da/Apnctest.jpg" id="logo" name="logo" height="150" width="300" alt="" /></html>This construct uses the xylA facing promoter that does not include the CRP binding region.
 

Latest revision as of 21:09, 26 October 2007

Initial Setup