PennState/Project/Dosimeter/InitialTest

From 2007.igem.org

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'''Promoter Test Constructs'''
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'''Dosimeter Test Constructs'''
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To test each of the four xyl promoters, each is placed in a construct followed by a ribosome binding site, the reporter green florescent protein, and a terminator. The strength of each is measured by gfp emission.
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To test the dosimeter, the assembled lambda region is placed in a plasmid preceding a ribosome binding site, the reporter green florescent protein, and a terminator. The promoter's sensitivity to radiation can be readily tested with flow cell cytometry and fluoresence plate reading
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<html><img src="http://openwetware.org/images/5/54/Dosimeter_Test_Construct.jpg" id="logo" name="logo" height="128" width="800" alt="" /></html>This construct uses the xylF and xylA facing promoters that includes the CRP-cAMP binding region.
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<html><img src="http://openwetware.org/images/5/54/Dosimeter_Test_Construct.jpg" id="logo" name="logo" height="128" width="800" alt="" /></html>
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This construct uses the lambda operator to express GFP when induced by radiation.

Revision as of 22:36, 26 October 2007

Dosimeter Test Constructs

To test the dosimeter, the assembled lambda region is placed in a plasmid preceding a ribosome binding site, the reporter green florescent protein, and a terminator. The promoter's sensitivity to radiation can be readily tested with flow cell cytometry and fluoresence plate reading


This construct uses the lambda operator to express GFP when induced by radiation.

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