PennState/Project/Dosimeter/InitialTest
From 2007.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
<html><img src="http://openwetware.org/images/5/54/Dosimeter_Test_Construct.jpg" id="logo" name="logo" height="128" width="800" alt="" /><br></html> | <html><img src="http://openwetware.org/images/5/54/Dosimeter_Test_Construct.jpg" id="logo" name="logo" height="128" width="800" alt="" /><br></html> | ||
This construct uses the lambda operator to express GFP when induced by radiation. | This construct uses the lambda operator to express GFP when induced by radiation. | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
<html> | <html> | ||
Revision as of 22:55, 26 October 2007
Dosimeter Test Construct
To test the dosimeter, the assembled lambda region is placed in a plasmid preceding a ribosome binding site, the reporter green florescent protein, and a terminator. The promoter's sensitivity to radiation can be readily tested with flow cell cytometry and fluoresence plate reading
This construct uses the lambda operator to express GFP when induced by radiation.
