Prairie View

From 2007.igem.org

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(Student Profiles)
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== Project ==
== Project ==
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'''Title''' 
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''Development of a Biosensor Device for Detection of Several Metals Simultaneously''
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*'''Abstract'''
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Assembling parts for developing microbial biosensors for detection of toxic metals and for organic compounds.
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*'''Objective'''
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Develop and standardize microbial biosensors that respond specifically to toxic metals (nickel, vanadium, iron) simultaneously at different concentrations.
== Project Planning Journal ==
== Project Planning Journal ==

Revision as of 19:11, 26 July 2007

Contents

2007 IGEM Team

Advisors

Dr Raul Cuero,(PhD)

DRcuero.jpg

Dr. Raul Cuero, (PhD), Microbiologist, Distinguished Professor, Research Leader, Scientist and Contact Person for Prairie View A&M University iGEM 2007 group. Dr Cuero holds 8 scientific inventions and more than 90 scientific publications. He is currently working on fungal toxins at the molecular level, and on biogenesis. He is also the author of the book "Triumph and Survival".

Email: olimpa@aol.com

www.raulcuerobiotech.com


Student Profiles

Aziza | Laina | Princess | Sehnaz | Terry

Project

Title Development of a Biosensor Device for Detection of Several Metals Simultaneously

  • Abstract

Assembling parts for developing microbial biosensors for detection of toxic metals and for organic compounds.

  • Objective

Develop and standardize microbial biosensors that respond specifically to toxic metals (nickel, vanadium, iron) simultaneously at different concentrations.

Project Planning Journal

June

---No entry---

July

  1. Growth and Genomic DNA Determination of M. luteus
  2. Growth and Genomic DNA Determination of A. ferroxidons
  3. Isolation of plasmids from MIT, use of PCR
  4. Construction of competent cell M. luteus
  5. Assemble new synthesized sequences for iron gene expression and lux genes
  6. Look up the flourescent protein for changes with minimal of three types of proteins

References

  1. Cuero, R, Oullet T. Metal ions modulate gene expression and accumulation of the mycotoxins aflatoxin and zearalenone.J Appl Microbiol. 2005;98(3):598-605.
  2. Holmes, D.S. Dubey, U.K and Gongolli, 1993. Development of Biosensor for the detection of Mercury andCopper Ions.Environ, Geochem. Health 16: 229-233.
  3. Leonard G. Davis, Mark D. Dibner, James F Battey. Basic Methods in Molecular Biology. Elsevier Science Publishers.1986, New York, Amsterdam and London.
  4. Lovley, DR (2003). "Cleaning up with genomics: applying molecular biology to bioremediation". NATURE REVIEWS. MICROBIOLOGY. 1 (1): 35–44. PMID 15040178. Holmes et al 1993b. Holmes D.S et al, 1993. Development of Biosensor to Measure Metal Ions
  5. Bioavailability in mining and metal waste.pp659-666. In Biohydrometallurgical Technologies. Vol. II. A.E Torma, M.L Apel and C.L Brierle,TMS, Warrendale, PA,USA.Eds.