From 2007.igem.org

To create our circuite in yeast cell, we have to integrate '2XORE' promoter instead of Pho4 endogenous promoter

  First we cloned 2XORE into Hind-Bam E.R. site of p2UG25 plasmid that has natMX4 resistence, so we create a '2XORE-natMX4' cassette,
  that after we can integrate in the Pho4's ORF.

2XORE Fv Hind 2XORE Rv Bam

2XOREint Fv 2XOREint Rv