Princeton/lab/experimentation
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Revision as of 21:55, 26 October 2007
Contents |
Experimentation
Protocols
For reliability, a majority of our actual experimentation has followed protocols provided by the supplier of relevant projects, including enzymes (NEB) and kits (Qiagen). The Qiagen maxiprep kit was replaced with a Bio-Rad kit to obtain higher yields with lower processing times.
Please consult the relevant vendors for protocol documents.
Assembly Line Flow
Bio-Brick Creation
Design Primers
PCR
Run Gel
Gel Extraction and determine concentrations
Digestion with Restriction Enzymes
PCR purification and determine concentrations
Ligation