Princeton/lab/experimentation
From 2007.igem.org
(Difference between revisions)
(→Bio-Brick Creation) |
(→Bio-Brick Creation) |
||
Line 16: | Line 16: | ||
Run Gel | Run Gel | ||
+ | |||
+ | - Check Primer concentration | ||
+ | - Check Primer design | ||
Gel Extraction and determine concentrations | Gel Extraction and determine concentrations |
Revision as of 21:58, 26 October 2007
Contents |
Experimentation
Protocols
For reliability, a majority of our actual experimentation has followed protocols provided by the supplier of relevant projects, including enzymes (NEB) and kits (Qiagen). The Qiagen maxiprep kit was replaced with a Bio-Rad kit to obtain higher yields with lower processing times.
Please consult the relevant vendors for protocol documents.
Assembly Line Flow
Bio-Brick Creation
Design Primers
PCR
Run Gel
- Check Primer concentration - Check Primer design
Gel Extraction and determine concentrations
Digestion with Restriction Enzymes
PCR purification and determine concentrations
Ligation