http://2007.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=20&target=CelineYZ&year=&month=2007.igem.org - User contributions [en]2024-03-28T22:27:45ZFrom 2007.igem.orgMediaWiki 1.16.5http://2007.igem.org/wiki/index.php/Celine_ZengCeline Zeng2008-04-08T03:47:58Z<p>CelineYZ: </p>
<hr />
<div>Celine is in her third year of Specialization Pharmacology. She is interested in technology commercialization and translational research; global health; international standards on traditional herbal Chinese medicine. Celine enjoys traveling, yoga, dancing, reading and swimming. She has also worked as a summer student at the Cross Cancer Institute. She is also proud to be a ButaNerd. <br><br />
<br />
Back to [[Alberta]]<br><br />
Back to [[Alberta/Members]]</div>CelineYZhttp://2007.igem.org/wiki/index.php/Celine_ZengCeline Zeng2007-12-29T02:28:45Z<p>CelineYZ: </p>
<hr />
<div>Celine is in her third year of Honors Pharmacology. She hopes to get into an Industrial Internship Program in the pharmaceutical industry after third year. After graduation, she will eventually pursue a PhD in Biomedical Sciences or Chemical Biology. Celine is interested in technology commercialization and translational research. She is also interested in influencing policy on the quality and efficacy control of traditional herbal Chinese medicine on a global level. Her ultimate career goal is to establish her own consulting and research firm ''Zenomics''. <br />
<br />
Outside of the lab, Celine enjoys traveling, yoga, dancing, reading and swimming. She is also working as a summer student at the Cross Cancer Institute. She is also proud to be a ButaNerd. <br><br />
<br />
Back to [[Alberta]]<br><br />
Back to [[Alberta/Members]]</div>CelineYZhttp://2007.igem.org/wiki/index.php/AlbertaAlberta2007-10-27T04:48:37Z<p>CelineYZ: /* '''The Project: Plan B''' */</p>
<hr />
<div><center>[[Image: headbanner.jpg]] <br />
<br><br />
''The Offical Wiki of the 2007 University of Alberta iGEM Team'' <br><br />
''Edmonton, Alberta, Canada </center>''<br />
<br />
== '''Background: Biofuels'''==<br />
<br />
<br />
With growing concerns of global greenhouse gasses, the global energy market is in a continual push for the development of renewable energy sources. Specifically, two fuels have dominated the media: biodiesel and ethanol. However, both of these fuels have shortcomings in terms of being a viable fuel source.<br />
<br />
<br />
Biodiesel is a fuel produced from the vegetable oils of crops that can be used in an engine system very similar to a traditional diesel engine. However, vegetable oils in crops only make up a small portion of the biomass of the plants, ultimately producing low yields of fuel per acre of crops. As such, it is more economically sound to use these resources for food production.<br />
<br />
<br />
There has been huge attention to the use of ethanol in the typical auto cycle engine. In many places it is already being blended with gasoline to create a hybrid fuel source. However, running an engine on pure ethanol is beset by several major obstacles. Firstly, ethanol is miscible with water at any concentration, which creates long term storage corrosion issues. Secondly, ethanol engines must be designed to expect water vapor unlike their gasoline counterparts. Ethanol also has significantly different thermodynamic properties than gasoline such as a lower energy density and different vapor properties, which would reduce the economical advantage of using ethanol as a primary fuels source.<br />
<br />
<br />
We propose using a different fuel source to eventually replace gasoline, butanol. Butanol is a superior to ethanol as a replacement for petroleum gasoline. With a low vapor pressure, high energy density, and a gasoline-like octane rating, it can be blended into existing gasoline at much higher proportions than ethanol without compromising performance, mileage, organic pollution standards. Blending butanol with gasoline also prevents major modifications of the fuel-air ratio and modifications to the fuel system. Butanol also is immiscible with water at concentrations higher than 7%, alleviating storage concerns as well as offering the possiblity of phase separation which would realize huge cost savings in terms of production.<br />
<br />
<br />
More information on the summary of biofuels viability and the inspiration to our project can be found '''[[Alberta/background|here]]'''.<br />
<br />
<br />
The Butanerds wanted to further evaluate the use of butanol as a fuel in standard spark ignition engines. With the assistance of The University of Alberta's Engine Control Lab in Mechanical Engineering we were able to run a peak power test comparing butanol to iso-octane, a standard test measurement. Each fuel was burned at a stoichiometric air to fuel ratio at 4 different angular speeds. The experimental setup and results can be seen below.<br />
<br />
[[image: alberta_engine1.jpeg|thumb|left|275px| Engine Test Setup Front View]]<br />
[[image: alberta_engine2.jpeg|thumb|left|275px| Engine Test Setup Side View]]<br />
[[image: alberta_enginechart2.jpeg|thumb|left|275px| Peak Power vs. RPM Experimental Data]]<br />
<br><br />
<br />
== '''The Project: Plan B''' ==<br />
<br />
We propose the use of butanol as the leading biofuel for use in internal combustion engines. Specifically, we intend to genetically engineer ''Escherichia coli'' bacteria to convert biomass into butanol for use as an energy source. This will be accomplished by introducing the genes responsible for butanol production from ''Clostridium acetobutylicum'' (i.e. endogenous butanoate pathway) into ''E. coli''. Furthermore, we hope to increase ''E. coli'''s tolerance to solvents such as butanol.<br />
<br />
I: Transforming genes in ''C. acetobutylicum'''s butanoate pathway into ''E.coli''<br><br />
Genes encoding the enzymes in the ''C. acetobutylicum'' butanoate metabolism pathway were identified using the KEGG database (KEGG number-ca00650: http://www.kegg.com/dbget-bin/www_bget?path:cac00650). Our cloning stragegy is to incorporate all the genes in a single operon with respective inducible promoter and ribosome binding site in a plasmid. Such construct enables easy transformation of multiple genes simultaneously into ''E.coli'' and allows the coordinated expression of genes within the operon. <br><br />
<br />
After receiving the commercially synthesized coding sequences of the genes of interest, the coding sequences are restricted with proper BioBrick prefixes and suffixes out of the original plasmid and cloned into B0034 (ribosome binding site). Double digest (Xba I and Spe I) as well as automated sequencing reactions are performed to verify the proper insertion of the coding sequences and the presence of a ribosome binding site at the 5' end of each coding sequence. <br><br />
<br />
The operon, shown below, consists of all of the genes in the pathway (except ''E. coli'''s thiolase). Note that we purposedly added a Histamine tag to the carboxy terminus of each gene product such that we could use Nickel-NTA column to purify the tagged proteins and subsequently analyze protein expression and enzymatic activity.<br />
[[image: alberta_butanol_Operon.jpeg|thumb|center|400px|The Butanol Operon]]<br />
<br />
II: Developing butanol tolerance with the mutagenic and toxic compound--Ethylnitrosourea ENU<br><br />
The concept is to make butanol-agar plates of various butanol concentrations and place an ENU disk in the centre. We expect two ring-shaped kill zones. The first where bacteria die due to the toxicity of ENU at the centre of the plate. The second around the outer edge of the butanol-agr plate where bacteria die due to butanol toxicity. In between these two zones we hope to see some cells that would be mutated in a benifical way to render them resistant to butanol.<br />
<br />
[[image: alberta_butanolplate2.jpeg|400px|thumb|center|Proposed Butanol-ENU Plate for Mutagenisis of ''E. coli'']]<br />
<br />
<br />
Concurrently, we are investigating the possibiilty of introducing the butanol production pathway into a photoautotrophic bacterium, ''Chlorobium tepidum''. ''C. tepidum'' is a green sulfur bacterium that is strictly anaerobic and uses sulfur compounds as terminal electron donors. ''C. tepidum'' is a moderately thermophillic bacterium, growing at 40 degrees Celsius (104 degrees Fahrenheit), and requires low light conditions for optimal growth. These bacteria grow well in a defined medium, utilizing the reverse/reductive tricarboxylic acid (TCA) cycle to build up carbohydrates from carbon dioxide. In addition, the genome of this bacterium has been completely sequenced. For a more comprehensive overview of ''Chlorobium tepidum'' go to [[http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html ''here'']]. Images below from http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html<br />
<br />
[[Image:spacer.jpeg|left]][[Image:Alberta_micrograph.gif|thumb|300px|left|Figure 1:''Chlorobium tepidum'' Transmission Micrograph Image]]<br />
[[Image:Alberta_gramstain.jpg|thumb|330px|left|Figure 2:''Chlorobium tepidum'' Standard Light Microscope Image]]<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br />
<br />
The advantage of manipulating this organism for butanol production is a matter of energy input and output. Rather than having to utilize vast amounts of food stocks, such as grains or sugars, a photoautotroph will fix carbon dioxide into the complex carbohydrates required for butanol production. Theoretically, if the only fuel on our planet was butanol, and it was produced in this manner, there would be little net carbon dioxide produced.<br />
<br />
[[image: alberta_carboncycle1.jpeg|thumb|300px|center| Propose Closed Carbon-butanol Cycle]]<br />
<br />
== '''Modeling Efforts''' ==<br />
<br />
Initially our plan to model the efficiency of the butanol production hinged on developing a complete model for the entire glucose pathway in an ''E. coli'' cell using Michaelis-Menten kinetics. After further review, this proved to be beyond the scope of our project's timeline due both to the large number of species and reactions present in the system (as well as their non-linear behavior) and the relative difficulty in finding experimental kinetic information for some of the reactions in our pathway. <br> <br />
<br />
<br />
Therefore, to obtain a rough "first-order" approximation for the behavior of the pathway, we considered three factors:<br />
<br />
1) Stoichiometric factors (both redox and carbon)<br><br />
2) Compared the relative production rates of the various end products of the glucose pathway in ''E. coli'' as found in previous experimentation to the production rates of butanol in ''C. acetobutylicum''. <br><br />
3) We have also a fuel E. coli Stoichometric Matrix, and intend to stoichiometrically model the system in order to optimize butanol production once the operon is in the system.<br />
<br />
<br />
From these factors we can obtain an indicator of how likely butanol will be produced.<br />
<br><br />
<br />
In order to further develop the system, we hope a full metabolic stochiometric model can be eventually realized for this project. This would allow optimization of the system in order to maximize the flux through the butanol pathway.<br />
<br />
== '''Project Timeline''' ==<br />
<br />
[[image: Alberta_gantt.jpeg|thumb|400px|center|[[Project Gantt Chart (click here to enlarge)]]]]<br />
<br />
The GANTT Chart Above details the schedule for the lab work that contributed to Plan B. Beginning in July and finishing with our final work in October, the schedule includes details on our different methods to compose an operon that would meet our objectives. In order to read the GANTT Chart for the Lab work of Plan B, please refer to the legend below.<br />
<br />
'''Legend: ''' <br><br />
Benny - Butyryl CoA dehydrogenase<br><br />
Enny - Enoyl CoA hydratase<br><br />
Buddy - Butanol dehydrogenase<br><br />
Betty - Beta-hydraoxy butyryl CoA dehydrogenase<br><br />
Deisel Blaze - Butyraldehyde dehydrogenase<br><br />
<br />
== '''Lab Book and Calendar''' ==<br />
[[image: Alberta_butapipettips.jpg]][[image: Alberta_arrow.jpg]] [[image: Alberta_Labbook.jpg]] <br><br />
<br />
Butanerd Event Calendar can be found below:<br />
<br />
[http://www.ualberta.ca/~mjl3/UofAIgemProtocols.pdf The Lab Protocols]<br />
<br />
[[Alberta/Calender/July|July 2007]]<br />
<br />
[[Alberta/Calender/August|August 2007]]<br />
<br />
[[Alberta/Calender/September|September 2007]]<br />
<br />
[[Alberta/Calender/October|October 2007]]<br />
<br />
Meetings (Agenda's, Minutes, Action Items):<br />
<br />
[[Alberta/Calender/Meetings|Meeting Information]]<br />
<br />
<br />
Online Form:<br />
<br />
'''[http://butanerds.myfreeforum.org The Butanerd Online Forum]''' is up and running. Feel free to post and check for posts here. Make sure you register!<br />
<br />
== '''The Team''' ==<br />
<br />
<center>[[Image: Alberta_team_new.jpg]] </center><br />
<br><br />
<br />
Our team has a rich background in biology, biochemistry and engineering. To compliment our diversity we also have advisors who have a wealth of knowledge in research and applications of genetic engineering. For more information about the group, check out the '''[[Alberta/Members|University of Alberta's iGEM Team Members Page]]'''.<br />
<br />
Our University of Alberta Student Group Constitution can be found '''[[alberta/constitution|here]]'''<br />
<br />
== '''Edmonton''' ==<br />
<br />
Welcome to Edmonton!<br />
<br />
<center>[[Image: Alberta_edmonton.jpg]]</center><br />
<br><br />
<br />
For more on the city of Edmonton click '''[http://www.ualberta.ca/~mjl3/About.html here]'''.<br />
<br />
== '''Sponsors''' ==<br />
<br />
We would like to thank all of our sponsers for their gracious support and our advisors for invaluble advise.<br />
<br />
<br />
<b>Major Sponsors</b><br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors1.gif]]<br />
|}<br />
<br />
<br />
'''Sponsors'''<br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors2.gif]]<br />
|}<br />
<br />
'''Advice'''<br />
<br />
<br />
'''Andrew Hessel''' - ''Alberta Ingenuity Mentor''<br><br />
'''Dr. Jonathan Dennis''' - ''University of Alberta''<br><br />
'''Dr. Perrin Beatty''' - ''University of Alberta''<br><br />
'''Dr. David Bressler''' - ''University of Alberta''<br><br />
'''Dr. Charles Lucy''' - ''University of Alberta''<br><br />
'''Dr. Gregory Kiema''' - ''University of Alberta''<br><br />
'''Dr. Mark S. Peppler''' - ''University of Alberta''<br><br />
'''Dr. James Harynuk''' - ''University of Alberta''<br><br />
'''Dr. Federick West'''-''University of Alberta''<br><br />
'''Dr. Todd Lowary'''-''University of Alberta''<br><br />
'''Desiree Schell''' - ''University of Alberta CJSR''<br><br />
'''Dr. Jeff Fuller''' - ''University of Alberta/Capital Health''<br><br />
'''Dr. Julia Foght''' - ''University of Alberta''<br><br />
'''Dr. David Checkel''' - ''University of Alberta''<br><br />
'''Adrian Audiet''' - ''University of Alberta''<br><br />
'''Dr. Koch''' - ''University of Alberta''<br><br />
'''Dr. Donald Bryant''' - ''Pennsylvania State''<br><br />
'''Dr. Gaozhong Shen''' - ''Pennsylvania State''<br><br />
'''Amaya Garcia''' - ''Pennsylvania State''<br><br />
<br />
<br />
A list of experimental supplies we need can be found '''[[supplies|here]]'''<br />
<br />
== '''External Links''' ==<br />
<br />
[http://www.albertaingenuity.ca/ Alberta Ingenuity Fund]<br />
<br />
[http://www.ualberta.ca The University of Alberta Hompage]<br />
<br />
[http://www.mece.ualberta.ca/~ckoch/ Dr. Koch's Engine Lab]<br />
<br />
[http://www.systems-biology.org/cd Cell Designer Homepage]<br />
<br />
[http://www.biomodels.org Biomodels Home Page]</div>CelineYZhttp://2007.igem.org/wiki/index.php/AlbertaAlberta2007-10-27T04:45:48Z<p>CelineYZ: /* '''The Project: Plan B''' */</p>
<hr />
<div><center>[[Image: headbanner.jpg]] <br />
<br><br />
''The Offical Wiki of the 2007 University of Alberta iGEM Team'' <br><br />
''Edmonton, Alberta, Canada </center>''<br />
<br />
== '''Background: Biofuels'''==<br />
<br />
<br />
With growing concerns of global greenhouse gasses, the global energy market is in a continual push for the development of renewable energy sources. Specifically, two fuels have dominated the media: biodiesel and ethanol. However, both of these fuels have shortcomings in terms of being a viable fuel source.<br />
<br />
<br />
Biodiesel is a fuel produced from the vegetable oils of crops that can be used in an engine system very similar to a traditional diesel engine. However, vegetable oils in crops only make up a small portion of the biomass of the plants, ultimately producing low yields of fuel per acre of crops. As such, it is more economically sound to use these resources for food production.<br />
<br />
<br />
There has been huge attention to the use of ethanol in the typical auto cycle engine. In many places it is already being blended with gasoline to create a hybrid fuel source. However, running an engine on pure ethanol is beset by several major obstacles. Firstly, ethanol is miscible with water at any concentration, which creates long term storage corrosion issues. Secondly, ethanol engines must be designed to expect water vapor unlike their gasoline counterparts. Ethanol also has significantly different thermodynamic properties than gasoline such as a lower energy density and different vapor properties, which would reduce the economical advantage of using ethanol as a primary fuels source.<br />
<br />
<br />
We propose using a different fuel source to eventually replace gasoline, butanol. Butanol is a superior to ethanol as a replacement for petroleum gasoline. With a low vapor pressure, high energy density, and a gasoline-like octane rating, it can be blended into existing gasoline at much higher proportions than ethanol without compromising performance, mileage, organic pollution standards. Blending butanol with gasoline also prevents major modifications of the fuel-air ratio and modifications to the fuel system. Butanol also is immiscible with water at concentrations higher than 7%, alleviating storage concerns as well as offering the possiblity of phase separation which would realize huge cost savings in terms of production.<br />
<br />
<br />
More information on the summary of biofuels viability and the inspiration to our project can be found '''[[Alberta/background|here]]'''.<br />
<br />
<br />
The Butanerds wanted to further evaluate the use of butanol as a fuel in standard spark ignition engines. With the assistance of The University of Alberta's Engine Control Lab in Mechanical Engineering we were able to run a peak power test comparing butanol to iso-octane, a standard test measurement. Each fuel was burned at a stoichiometric air to fuel ratio at 4 different angular speeds. The experimental setup and results can be seen below.<br />
<br />
[[image: alberta_engine1.jpeg|thumb|left|275px| Engine Test Setup Front View]]<br />
[[image: alberta_engine2.jpeg|thumb|left|275px| Engine Test Setup Side View]]<br />
[[image: alberta_enginechart2.jpeg|thumb|left|275px| Peak Power vs. RPM Experimental Data]]<br />
<br><br />
<br />
== '''The Project: Plan B''' ==<br />
<br />
We propose the use of butanol as the leading biofuel for use in internal combustion engines. Specifically, we intend to genetically engineer ''Escherichia coli'' bacteria to convert biomass into butanol for use as an energy source. This will be accomplished by introducing the genes responsible for butanol production from ''Clostridium acetobutylicum'' (i.e. endogenous butanoate pathway) into ''E. coli''. Furthermore, we hope to increase ''E. coli'''s tolerance to solvents such as butanol.<br />
<br />
I: Transforming genes in ''C. acetobutylicum'''s butanoate pathway into ''E.coli''<br><br />
Genes encoding the enzymes in the ''C. acetobutylicum'' butanoate metabolism pathway were identified using the KEGG database (KEGG number-ca00650: http://www.kegg.com/dbget-bin/www_bget?path:cac00650). Our cloning stragegy is to incorporate all the genes in a single operon with respective inducible promoter and ribosome binding site in a plasmid. Such construct enables easy transformation of multiple genes simultaneously into ''E.coli'' and allows the coordinated expression of genes within the operon. <br><br />
<br />
After receiving the commercially synthesized coding sequences of the genes of interest, the coding sequences are restricted with proper BioBrick prefixes and suffixes out of the original plasmid and cloned into B0034 (ribosome binding site). Double digest (Xba I and Spe I) as well as automated sequencing reactions are performed to verify the proper insertion of the coding sequences and the presence of a ribosome binding site at the 5' end of each coding sequence. <br><br />
<br />
The operon, shown below, consists of all of the genes in the pathway (except ''E. coli'''s thiolase). Note that we purposedly added a Histamine tag to the carboxy terminus of each gene product such that we could use Nickel-NTA column to purify the tagged proteins and subsequently analyze protein expression and enzymatic activity.<br />
[[image: alberta_butanol_Operon.jpeg|thumb|center|400px|The Butanol Operon]]<br />
<br />
II: Developing butanol tolerance with the mutagenic and toxic compound--Ethylnitrosourea ENU<br><br />
The concept is to make butanol-agar plates of various butanol concentrations and place an ENU disk in the centre. We expect two ring-shaped kill zones. The first where bacteria die due to the toxicity of ENU at the centre of the plate. The second around the outer edge of the butanol-agr plate where bacteria die due to butanol toxicity. In between these two zones we hope to see some cells that would be mutated in a benifical way to render them resistant to butanol.<br />
<br />
[[image: alberta_butanolplate2.jpeg|400px|thumb|center|Proposed Butanol-ENU Plate for Mutagenisis of ''E. coli'']]<br />
<br />
<br />
Concurrently, we are investigating the use of a photoautotrophic bacterium, ''Chlorobium tepidum'' that we will also introduce butanol producing genes to. ''Chlorobium tepidum'' is a green sulfur bacterium that is strictly anaerobic and uses sulfur compounds as terminal electron donors. ''Chlorobium tepidum'' is a moderately thermophillic bacterium, growing at 40 degrees Celsius (104 degrees Fahrenheit), and requires low light conditions for optimal growth. These bacteria grow well in a defined medium, utilizing the reverse/reductive tricarboxylic acid (TCA) cycle to build up carbohydrates from carbon dioxide. For a more comprehensive overview of ''Chlorobium tepidum'' go to [[http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html ''here'']]. Images below from http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html<br />
<br />
[[Image:spacer.jpeg|left]][[Image:Alberta_micrograph.gif|thumb|300px|left|Figure 1:''Chlorobium tepidum'' Transmission Micrograph Image]]<br />
[[Image:Alberta_gramstain.jpg|thumb|330px|left|Figure 2:''Chlorobium tepidum'' Standard Light Microscope Image]]<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br />
<br />
The advantage of manipulating this organism for butanol production is a matter of energy input and output. Rather than having to utilize vast amounts of food stocks, such as grains or sugars, a photoautotroph will fix carbon dioxide into the complex carbohydrates required for butanol production. Theoretically, if the only fuel on our planet was butanol, and it was produced in this manner, there would be little net carbon dioxide produced.<br />
<br />
[[image: alberta_carboncycle1.jpeg|thumb|300px|center| Propose Closed Carbon-butanol Cycle]]<br />
<br />
== '''Modeling Efforts''' ==<br />
<br />
Initially our plan to model the efficiency of the butanol production hinged on developing a complete model for the entire glucose pathway in an ''E. coli'' cell using Michaelis-Menten kinetics. After further review, this proved to be beyond the scope of our project's timeline due both to the large number of species and reactions present in the system (as well as their non-linear behavior) and the relative difficulty in finding experimental kinetic information for some of the reactions in our pathway. <br> <br />
<br />
<br />
Therefore, to obtain a rough "first-order" approximation for the behavior of the pathway, we considered three factors:<br />
<br />
1) Stoichiometric factors (both redox and carbon)<br><br />
2) Compared the relative production rates of the various end products of the glucose pathway in ''E. coli'' as found in previous experimentation to the production rates of butanol in ''C. acetobutylicum''. <br><br />
3) We have also a fuel E. coli Stoichometric Matrix, and intend to stoichiometrically model the system in order to optimize butanol production once the operon is in the system.<br />
<br />
<br />
From these factors we can obtain an indicator of how likely butanol will be produced.<br />
<br><br />
<br />
In order to further develop the system, we hope a full metabolic stochiometric model can be eventually realized for this project. This would allow optimization of the system in order to maximize the flux through the butanol pathway.<br />
<br />
== '''Project Timeline''' ==<br />
<br />
[[image: Alberta_gantt.jpeg|thumb|400px|center|[[Project Gantt Chart (click here to enlarge)]]]]<br />
<br />
The GANTT Chart Above details the schedule for the lab work that contributed to Plan B. Beginning in July and finishing with our final work in October, the schedule includes details on our different methods to compose an operon that would meet our objectives. In order to read the GANTT Chart for the Lab work of Plan B, please refer to the legend below.<br />
<br />
'''Legend: ''' <br><br />
Benny - Butyryl CoA dehydrogenase<br><br />
Enny - Enoyl CoA hydratase<br><br />
Buddy - Butanol dehydrogenase<br><br />
Betty - Beta-hydraoxy butyryl CoA dehydrogenase<br><br />
Deisel Blaze - Butyraldehyde dehydrogenase<br><br />
<br />
== '''Lab Book and Calendar''' ==<br />
[[image: Alberta_butapipettips.jpg]][[image: Alberta_arrow.jpg]] [[image: Alberta_Labbook.jpg]] <br><br />
<br />
Butanerd Event Calendar can be found below:<br />
<br />
[http://www.ualberta.ca/~mjl3/UofAIgemProtocols.pdf The Lab Protocols]<br />
<br />
[[Alberta/Calender/July|July 2007]]<br />
<br />
[[Alberta/Calender/August|August 2007]]<br />
<br />
[[Alberta/Calender/September|September 2007]]<br />
<br />
[[Alberta/Calender/October|October 2007]]<br />
<br />
Meetings (Agenda's, Minutes, Action Items):<br />
<br />
[[Alberta/Calender/Meetings|Meeting Information]]<br />
<br />
<br />
Online Form:<br />
<br />
'''[http://butanerds.myfreeforum.org The Butanerd Online Forum]''' is up and running. Feel free to post and check for posts here. Make sure you register!<br />
<br />
== '''The Team''' ==<br />
<br />
<center>[[Image: Alberta_team_new.jpg]] </center><br />
<br><br />
<br />
Our team has a rich background in biology, biochemistry and engineering. To compliment our diversity we also have advisors who have a wealth of knowledge in research and applications of genetic engineering. For more information about the group, check out the '''[[Alberta/Members|University of Alberta's iGEM Team Members Page]]'''.<br />
<br />
Our University of Alberta Student Group Constitution can be found '''[[alberta/constitution|here]]'''<br />
<br />
== '''Edmonton''' ==<br />
<br />
Welcome to Edmonton!<br />
<br />
<center>[[Image: Alberta_edmonton.jpg]]</center><br />
<br><br />
<br />
For more on the city of Edmonton click '''[http://www.ualberta.ca/~mjl3/About.html here]'''.<br />
<br />
== '''Sponsors''' ==<br />
<br />
We would like to thank all of our sponsers for their gracious support and our advisors for invaluble advise.<br />
<br />
<br />
<b>Major Sponsors</b><br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors1.gif]]<br />
|}<br />
<br />
<br />
'''Sponsors'''<br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors2.gif]]<br />
|}<br />
<br />
'''Advice'''<br />
<br />
<br />
'''Andrew Hessel''' - ''Alberta Ingenuity Mentor''<br><br />
'''Dr. Jonathan Dennis''' - ''University of Alberta''<br><br />
'''Dr. Perrin Beatty''' - ''University of Alberta''<br><br />
'''Dr. David Bressler''' - ''University of Alberta''<br><br />
'''Dr. Charles Lucy''' - ''University of Alberta''<br><br />
'''Dr. Gregory Kiema''' - ''University of Alberta''<br><br />
'''Dr. Mark S. Peppler''' - ''University of Alberta''<br><br />
'''Dr. James Harynuk''' - ''University of Alberta''<br><br />
'''Dr. Federick West'''-''University of Alberta''<br><br />
'''Dr. Todd Lowary'''-''University of Alberta''<br><br />
'''Desiree Schell''' - ''University of Alberta CJSR''<br><br />
'''Dr. Jeff Fuller''' - ''University of Alberta/Capital Health''<br><br />
'''Dr. Julia Foght''' - ''University of Alberta''<br><br />
'''Dr. David Checkel''' - ''University of Alberta''<br><br />
'''Adrian Audiet''' - ''University of Alberta''<br><br />
'''Dr. Koch''' - ''University of Alberta''<br><br />
'''Dr. Donald Bryant''' - ''Pennsylvania State''<br><br />
'''Dr. Gaozhong Shen''' - ''Pennsylvania State''<br><br />
'''Amaya Garcia''' - ''Pennsylvania State''<br><br />
<br />
<br />
A list of experimental supplies we need can be found '''[[supplies|here]]'''<br />
<br />
== '''External Links''' ==<br />
<br />
[http://www.albertaingenuity.ca/ Alberta Ingenuity Fund]<br />
<br />
[http://www.ualberta.ca The University of Alberta Hompage]<br />
<br />
[http://www.mece.ualberta.ca/~ckoch/ Dr. Koch's Engine Lab]<br />
<br />
[http://www.systems-biology.org/cd Cell Designer Homepage]<br />
<br />
[http://www.biomodels.org Biomodels Home Page]</div>CelineYZhttp://2007.igem.org/wiki/index.php/AlbertaAlberta2007-10-27T04:39:05Z<p>CelineYZ: /* '''The Project: Plan B''' */</p>
<hr />
<div><center>[[Image: headbanner.jpg]] <br />
<br><br />
''The Offical Wiki of the 2007 University of Alberta iGEM Team'' <br><br />
''Edmonton, Alberta, Canada </center>''<br />
<br />
== '''Background: Biofuels'''==<br />
<br />
<br />
With growing concerns of global greenhouse gasses, the global energy market is in a continual push for the development of renewable energy sources. Specifically, two fuels have dominated the media: biodiesel and ethanol. However, both of these fuels have shortcomings in terms of being a viable fuel source.<br />
<br />
<br />
Biodiesel is a fuel produced from the vegetable oils of crops that can be used in an engine system very similar to a traditional diesel engine. However, vegetable oils in crops only make up a small portion of the biomass of the plants, ultimately producing low yields of fuel per acre of crops. As such, it is more economically sound to use these resources for food production.<br />
<br />
<br />
There has been huge attention to the use of ethanol in the typical auto cycle engine. In many places it is already being blended with gasoline to create a hybrid fuel source. However, running an engine on pure ethanol is beset by several major obstacles. Firstly, ethanol is miscible with water at any concentration, which creates long term storage corrosion issues. Secondly, ethanol engines must be designed to expect water vapor unlike their gasoline counterparts. Ethanol also has significantly different thermodynamic properties than gasoline such as a lower energy density and different vapor properties, which would reduce the economical advantage of using ethanol as a primary fuels source.<br />
<br />
<br />
We propose using a different fuel source to eventually replace gasoline, butanol. Butanol is a superior to ethanol as a replacement for petroleum gasoline. With a low vapor pressure, high energy density, and a gasoline-like octane rating, it can be blended into existing gasoline at much higher proportions than ethanol without compromising performance, mileage, organic pollution standards. Blending butanol with gasoline also prevents major modifications of the fuel-air ratio and modifications to the fuel system. Butanol also is immiscible with water at concentrations higher than 7%, alleviating storage concerns as well as offering the possiblity of phase separation which would realize huge cost savings in terms of production.<br />
<br />
<br />
More information on the summary of biofuels viability and the inspiration to our project can be found '''[[Alberta/background|here]]'''.<br />
<br />
<br />
The Butanerds wanted to further evaluate the use of butanol as a fuel in standard spark ignition engines. With the assistance of The University of Alberta's Engine Control Lab in Mechanical Engineering we were able to run a peak power test comparing butanol to iso-octane, a standard test measurement. Each fuel was burned at a stoichiometric air to fuel ratio at 4 different angular speeds. The experimental setup and results can be seen below.<br />
<br />
[[image: alberta_engine1.jpeg|thumb|left|275px| Engine Test Setup Front View]]<br />
[[image: alberta_engine2.jpeg|thumb|left|275px| Engine Test Setup Side View]]<br />
[[image: alberta_enginechart2.jpeg|thumb|left|275px| Peak Power vs. RPM Experimental Data]]<br />
<br><br />
<br />
== '''The Project: Plan B''' ==<br />
<br />
We propose the use of butanol as the leading biofuel for use in internal combustion engines. Specifically, we intend to genetically engineer ''Escherichia coli'' bacteria to convert biomass into butanol for use as an energy source. This will be accomplished by introducing the genes responsible for butanol production from ''Clostridium acetobutylicum'' (i.e. endogenous butanoate pathway) into ''E. coli''. Furthermore, we hope to increase ''E. coli'''s tolerance to solvents such as butanol.<br />
<br />
I: Transforming genes in ''C. acetobutylicum'''s butanoate pathway into ''E.coli''<br><br />
Genes encoding the enzymes in the ''C. acetobutylicum'' butanoate metabolism pathway were identified using the KEGG database (KEGG number-ca00650: http://www.kegg.com/dbget-bin/www_bget?path:cac00650). Our cloning stragegy is to incorporate all the genes in a single operon with respective inducible promoter and ribosome binding site in a plasmid. Such construct enables easy transformation of multiple genes simultaneously into ''E.coli'' and allows the coordinated expression of genes within the operon. <br><br />
<br />
After receiving the commercially synthesized coding sequences of the genes of interest, the coding sequences are restricted with proper BioBrick prefixes and suffixes out of the original plasmid and cloned into B0034 (ribosome binding site). Double digest (Xba I and Spe I) as well as automated sequencing reactions were performed to verify the proper insertion of the coding sequences and the presence of a ribosome binding site at the 5' end of each coding sequence. <br><br />
<br />
The operon, shown below, consists of all of the genes in the pathway (except ''E. coli'''sthiolase). Note that we purposedly added a Histamine tag to the carboxy terminus of each gene product such that we could use Nickel-NTA column to purify the individual proteins and analyze protein expression and activity.<br />
[[image: alberta_butanol_Operon.jpeg|thumb|center|400px|The Butanol Operon]]<br />
<br />
II: Develop Butanol Tolerance with mutagenic and toxic compound Ethylnitrosourea ENU.<br><br />
The concept is to make butanol agar plates of various butanol concentrations and place an ENU disk in the centre. We expect there will be two kill zones. The first where bacteria die due to the toxicity of ENU at the centre of the plate. The second around the outer ring of the butanol plate where bacteria die due to butanol toxicity. In between these two zones we hope to see some cells that would be mutated in a benifical way to allow them to survive in the butanol.<br />
<br />
[[image: alberta_butanolplate2.jpeg|400px|thumb|center|Proposed Butanol-ENU Plate for Mutagenisis of ''E. coli'']]<br />
<br />
<br />
Concurrently, we are investigating the use of a photoautotrophic bacterium, ''Chlorobium tepidum'' that we will also introduce butanol producing genes to. ''Chlorobium tepidum'' is a green sulfur bacterium that is strictly anaerobic and uses sulfur compounds as terminal electron donors. ''Chlorobium tepidum'' is a moderately thermophillic bacterium, growing at 40 degrees Celsius (104 degrees Fahrenheit), and requires low light conditions for optimal growth. These bacteria grow well in a defined medium, utilizing the reverse/reductive tricarboxylic acid (TCA) cycle to build up carbohydrates from carbon dioxide. For a more comprehensive overview of ''Chlorobium tepidum'' go to [[http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html ''here'']]. Images below from http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html<br />
<br />
[[Image:spacer.jpeg|left]][[Image:Alberta_micrograph.gif|thumb|300px|left|Figure 1:''Chlorobium tepidum'' Transmission Micrograph Image]]<br />
[[Image:Alberta_gramstain.jpg|thumb|330px|left|Figure 2:''Chlorobium tepidum'' Standard Light Microscope Image]]<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br />
<br />
The advantage of manipulating this organism for butanol production is a matter of energy input and output. Rather than having to utilize vast amounts of food stocks, such as grains or sugars, a photoautotroph will fix carbon dioxide into the complex carbohydrates required for butanol production. Theoretically, if the only fuel on our planet was butanol, and it was produced in this manner, there would be little net carbon dioxide produced.<br />
<br />
[[image: alberta_carboncycle1.jpeg|thumb|300px|center| Propose Closed Carbon-butanol Cycle]]<br />
<br />
== '''Modeling Efforts''' ==<br />
<br />
Initially our plan to model the efficiency of the butanol production hinged on developing a complete model for the entire glucose pathway in an ''E. coli'' cell using Michaelis-Menten kinetics. After further review, this proved to be beyond the scope of our project's timeline due both to the large number of species and reactions present in the system (as well as their non-linear behavior) and the relative difficulty in finding experimental kinetic information for some of the reactions in our pathway. <br> <br />
<br />
<br />
Therefore, to obtain a rough "first-order" approximation for the behavior of the pathway, we considered three factors:<br />
<br />
1) Stoichiometric factors (both redox and carbon)<br><br />
2) Compared the relative production rates of the various end products of the glucose pathway in ''E. coli'' as found in previous experimentation to the production rates of butanol in ''C. acetobutylicum''. <br><br />
3) We have also a fuel E. coli Stoichometric Matrix, and intend to stoichiometrically model the system in order to optimize butanol production once the operon is in the system.<br />
<br />
<br />
From these factors we can obtain an indicator of how likely butanol will be produced.<br />
<br><br />
<br />
In order to further develop the system, we hope a full metabolic stochiometric model can be eventually realized for this project. This would allow optimization of the system in order to maximize the flux through the butanol pathway.<br />
<br />
== '''Project Timeline''' ==<br />
<br />
[[image: Alberta_gantt.jpeg|thumb|400px|center|[[Project Gantt Chart (click here to enlarge)]]]]<br />
<br />
The GANTT Chart Above details the schedule for the lab work that contributed to Plan B. Beginning in July and finishing with our final work in October, the schedule includes details on our different methods to compose an operon that would meet our objectives. In order to read the GANTT Chart for the Lab work of Plan B, please refer to the legend below.<br />
<br />
'''Legend: ''' <br><br />
Benny - Butyryl CoA dehydrogenase<br><br />
Enny - Enoyl CoA hydratase<br><br />
Buddy - Butanol dehydrogenase<br><br />
Betty - Beta-hydraoxy butyryl CoA dehydrogenase<br><br />
Deisel Blaze - Butyraldehyde dehydrogenase<br><br />
<br />
== '''Lab Book and Calendar''' ==<br />
[[image: Alberta_butapipettips.jpg]][[image: Alberta_arrow.jpg]] [[image: Alberta_Labbook.jpg]] <br><br />
<br />
Butanerd Event Calendar can be found below:<br />
<br />
[http://www.ualberta.ca/~mjl3/UofAIgemProtocols.pdf The Lab Protocols]<br />
<br />
[[Alberta/Calender/July|July 2007]]<br />
<br />
[[Alberta/Calender/August|August 2007]]<br />
<br />
[[Alberta/Calender/September|September 2007]]<br />
<br />
[[Alberta/Calender/October|October 2007]]<br />
<br />
Meetings (Agenda's, Minutes, Action Items):<br />
<br />
[[Alberta/Calender/Meetings|Meeting Information]]<br />
<br />
<br />
Online Form:<br />
<br />
'''[http://butanerds.myfreeforum.org The Butanerd Online Forum]''' is up and running. Feel free to post and check for posts here. Make sure you register!<br />
<br />
== '''The Team''' ==<br />
<br />
<center>[[Image: Alberta_team_new.jpg]] </center><br />
<br><br />
<br />
Our team has a rich background in biology, biochemistry and engineering. To compliment our diversity we also have advisors who have a wealth of knowledge in research and applications of genetic engineering. For more information about the group, check out the '''[[Alberta/Members|University of Alberta's iGEM Team Members Page]]'''.<br />
<br />
Our University of Alberta Student Group Constitution can be found '''[[alberta/constitution|here]]'''<br />
<br />
== '''Edmonton''' ==<br />
<br />
Welcome to Edmonton!<br />
<br />
<center>[[Image: Alberta_edmonton.jpg]]</center><br />
<br><br />
<br />
For more on the city of Edmonton click '''[http://www.ualberta.ca/~mjl3/About.html here]'''.<br />
<br />
== '''Sponsors''' ==<br />
<br />
We would like to thank all of our sponsers for their gracious support and our advisors for invaluble advise.<br />
<br />
<br />
<b>Major Sponsors</b><br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors1.gif]]<br />
|}<br />
<br />
<br />
'''Sponsors'''<br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors2.gif]]<br />
|}<br />
<br />
'''Advice'''<br />
<br />
<br />
'''Andrew Hessel''' - ''Alberta Ingenuity Mentor''<br><br />
'''Dr. Jonathan Dennis''' - ''University of Alberta''<br><br />
'''Dr. Perrin Beatty''' - ''University of Alberta''<br><br />
'''Dr. David Bressler''' - ''University of Alberta''<br><br />
'''Dr. Charles Lucy''' - ''University of Alberta''<br><br />
'''Dr. Gregory Kiema''' - ''University of Alberta''<br><br />
'''Dr. Mark S. Peppler''' - ''University of Alberta''<br><br />
'''Dr. James Harynuk''' - ''University of Alberta''<br><br />
'''Dr. Federick West'''-''University of Alberta''<br><br />
'''Dr. Todd Lowary'''-''University of Alberta''<br><br />
'''Desiree Schell''' - ''University of Alberta CJSR''<br><br />
'''Dr. Jeff Fuller''' - ''University of Alberta/Capital Health''<br><br />
'''Dr. Julia Foght''' - ''University of Alberta''<br><br />
'''Dr. David Checkel''' - ''University of Alberta''<br><br />
'''Adrian Audiet''' - ''University of Alberta''<br><br />
'''Dr. Koch''' - ''University of Alberta''<br><br />
'''Dr. Donald Bryant''' - ''Pennsylvania State''<br><br />
'''Dr. Gaozhong Shen''' - ''Pennsylvania State''<br><br />
'''Amaya Garcia''' - ''Pennsylvania State''<br><br />
<br />
<br />
A list of experimental supplies we need can be found '''[[supplies|here]]'''<br />
<br />
== '''External Links''' ==<br />
<br />
[http://www.albertaingenuity.ca/ Alberta Ingenuity Fund]<br />
<br />
[http://www.ualberta.ca The University of Alberta Hompage]<br />
<br />
[http://www.mece.ualberta.ca/~ckoch/ Dr. Koch's Engine Lab]<br />
<br />
[http://www.systems-biology.org/cd Cell Designer Homepage]<br />
<br />
[http://www.biomodels.org Biomodels Home Page]</div>CelineYZhttp://2007.igem.org/wiki/index.php/AlbertaAlberta2007-10-26T20:50:02Z<p>CelineYZ: /* '''The Project: Plan B''' */</p>
<hr />
<div><center>[[Image: headbanner.jpg]] <br />
<br><br />
''The Offical Wiki of the 2007 University of Alberta iGEM Team'' <br><br />
''Edmonton, Alberta, Canada </center>''<br />
<br />
== '''Background: Biofuels'''==<br />
<br />
<br />
With growing concerns of global greenhouse gasses, the global energy market is in a continual push for the development of renewable energy sources. Specifically, two fuels have dominated the media: biodiesel and ethanol. However, both of these fuels have shortcomings in terms of being a viable fuel source.<br />
<br />
<br />
Biodiesel is a fuel produced from the vegetable oils of crops that can be used in an engine system very similar to a traditional diesel engine. However, vegetable oils in crops only make up a small portion of the biomass of the plants, ultimately producing low yields of fuel per acre of crops. As such, it is more economically sound to use these resources for food production.<br />
<br />
<br />
There has been huge attention to the use of ethanol in the typical auto cycle engine. In many places it is already being blended with gasoline to create a hybrid fuel source. However, running an engine on pure ethanol is beset by several major obstacles. Firstly, ethanol is miscible with water at any concentration, which creates long term storage corrosion issues. Secondly, ethanol engines must be designed to expect water vapor unlike their gasoline counterparts. Ethanol also has significantly different thermodynamic properties than gasoline such as a lower energy density and different vapor properties, which would reduce the economical advantage of using ethanol as a primary fuels source.<br />
<br />
<br />
We propose using a different fuel source to eventually replace gasoline, butanol. Butanol is a superior to ethanol as a replacement for petroleum gasoline. With a low vapor pressure, high energy density, and a gasoline-like octane rating, it can be blended into existing gasoline at much higher proportions than ethanol without compromising performance, mileage, organic pollution standards. Blending butanol with gasoline also prevents major modifications of the fuel-air ratio and modifications to the fuel system. Butanol also is immiscible with water at concentrations higher than 7%, alleviating storage concerns as well as offering the possiblity of phase separation which would realize huge cost savings in terms of production.<br />
<br />
<br />
More information on the summary of biofuels viability and the inspiration to our project can be found '''[[Alberta/background|here]]'''.<br />
<br />
<br />
The Butanerds wanted to further evaluate the use of butanol as a fuel in standard spark ignition engines. With the assistance of The University of Alberta's Engine Control Lab in Mechanical Engineering we were able to run a peak power test comparing butanol to iso-octane, a standard test measurement. Each fuel was burned at a stoichometric air to fuel ratio at 4 different angular speeds. The experimental Setup and results can be seen below.<br />
<br />
[[image: alberta_engine1.jpeg|thumb|left|275px| Engine Test Setup Front View]]<br />
[[image: alberta_engine2.jpeg|thumb|left|275px| Engine Test Setup Side View]]<br />
[[image: alberta_enginechart2.jpeg|thumb|left|275px| Peak Power vs. RPM Experimental Data]]<br />
<br><br />
<br />
== '''The Project: Plan B''' ==<br />
<br />
We propose the use of butanol as the leading biofuel for use in internal combustion engines. Specifically, we intend to genetically engineer ''Escherichia coli'' bacteria to convert biomasses into butanol for use as an energy source. This will be accomplished by introducing the genes responsible for butanol production from ''Clostridium acetobutylicum''(i.e. endogenous butanoate pathway) into ''E. coli''. Furthermore, we hope to increase ''E. coli''s tolerance to solvents such as butanol.<br />
<br />
I: Transforming genes in ''C. acetobutylicum'' butanoate pathway into ''E.coli''<br><br />
Genes encoding the enzymes in the ''C.acetobutylicum'' butanoate metabolism pathway are identified using KEGG database (KEGG number-ca00650: http://www.kegg.com/dbget-bin/www_bget?path:cac00650). Our cloning stragegy is to incorporate all the genes in a single operon with respective inducible promoter and ribosome binding site in a plasmid. Such construct enables easy transformation of multiple genes simultaneously into ''E.coli'' and allows the coordinated expression of genes within the operon. <br><br />
<br />
After receiving the commercially synthesized coding sequences of the genes of interest, the coding sequences are restricted with proper BioBrick prefices and suffices out of the original plasmid and cloned into B0034 (ribosome binding site). Double digest (Xba I and Spe I) as well as automated sequencing reactions are performed to verify the proper insertion of the coding sequences and the presence of ribosome binding site at the 5' end of each coding sequence. <br><br />
<br />
The operon, shown below, consists of all of the genes of the pathway (except thiolase). Note that we purposedly added a Histamine tag to the carboxy terminus of each gene product such that we could use Nickel-NTA column to purify the individual proteins and analyze protein expression and activity.<br />
[[image: alberta_butanol_Operon.jpeg|thumb|center|400px|The Butanol Operon]]<br />
<br />
Objective 2: Develop Butanol Tolerance with mutagenic and toxic compound Ethylnitrosourea ENU.<br><br />
The concept is to make butanol agar plates of various butanol concentrations and place and ENU disk in the centre. We expect there will be two kill zones. The first where bacteria die due to the toxicity of ENU at the centre of the plate. The second around the outer ring of the butanol plate where bacteria die due to butanol toxicity. In between these two zones we hope to see some cells that would be mutated in a benifical way to allow them to survive in the butanol.<br />
<br />
[[image: alberta_butanolplate2.jpeg|400px|thumb|center|Proposed Butanol-ENU Plate for Mutagenisis of ''E. coli'']]<br />
<br />
<br />
Concurrently, we are investigating the use of a photoautotrophic bacterium, ''Chlorobium tepidum'' that we will also introduce butanol producing genes to. ''Chlorobium tepidum'' is a green sulfur bacterium that is strictly anaerobic and uses sulfur compounds as terminal electron donors. ''Chlorobium tepidum'' is a moderately thermophillic bacterium, growing at 40 degrees Celsius (104 degrees Fahrenheit), and requires low light conditions for optimal growth. These bacteria grow well in a defined medium, utilizing the reverse/reductive tricarboxylic acid (TCA) cycle to build up carbohydrates from carbon dioxide. For a more comprehensive overview of ''Chlorobium tepidum'' go to [[http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html ''here'']]. Images below from http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html<br />
<br />
[[Image:spacer.jpeg|left]][[Image:Alberta_micrograph.gif|thumb|300px|left|Figure 1:''Chlorobium tepidum'' Transmission Micrograph Image]]<br />
[[Image:Alberta_gramstain.jpg|thumb|330px|left|Figure 2:''Chlorobium tepidum'' Standard Light Microscope Image]]<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br />
<br />
The advantage of manipulating this organism for butanol production is a matter of energy input and output. Rather than having to utilize vast amounts of food stocks, such as grains or sugars, a photoautotroph will fix carbon dioxide into the complex carbohydrates required for butanol production. Theoretically, if the only fuel on our planet was butanol, and it was produced in this manner, there would be little net carbon dioxide produced.<br />
<br />
[[image: alberta_carboncycle1.jpeg|thumb|300px|center| Propose Closed Carbon-butanol Cycle]]<br />
<br />
== '''Modeling Efforts''' ==<br />
<br />
Initially our plan to model the efficiency of the butanol production hinged on developing a complete model for the entire glucose pathway in an ''E. coli'' cell using Michaelis-Menten kinetics. After further review, this proved to be beyond the scope of our project's timeline due both to the large number of species and reactions present in the system (as well as their non-linear behavior) and the relative difficulty in finding experimental kinetic information for some of the reactions in our pathway. <br> <br />
<br />
<br />
Therefore, to obtain a rough "first-order" approximation for the behavior of the pathway, we considered two factors:<br />
<br />
1) Stoichiometric factors (both redox and carbon)<br><br />
2) Compared the relative production rates of the various end products of the glucose pathway in ''E. coli'' as found in previous experimentation to the production rates of butanol in ''C. acetobutylicum''.<br />
3) We have also a fuel E. coli Stoichometric Matrix, and intend to stoichiometrically model the system in order to optimize butanol production once the operon is in the system.<br />
<br />
<br />
From these factors we can obtain an indicator of how likely butanol will be produced.<br />
<br><br />
<br />
In order to further develop the system, we hope a full metabolic stochiometric model can be eventually realized for this project. This would allow optimization of the system in order to maximize the flux through the butanol pathway.<br />
<br />
<br />
== '''Project Timeline''' ==<br />
<br />
[[image: Alberta_gantt.jpeg|thumb|400px|center|[[Project Gantt Chart (click here to enlarge)]]]]<br />
<br />
The GANTT Chart Above details the schedule for the lab work that contributed to Plan B. Beginning in July and finishing with our final work in October, the schedule includes details on our different methods to compose an operon that would meet our objectives. In order to read the GANTT Chart for the Lab work of Plan B, please refer to the legend below.<br />
<br />
'''Legend: ''' <br><br />
Benny - <br><br />
Enny - <br><br />
Buddy - <br><br />
Betty - <br><br />
Deisel Blaze - <br><br />
J61003 - <br><br />
B0034 - <br><br />
I0500 - <br><br />
Thiolase - <br><br />
BBDBE - Combination of genes in order of ...<br><br />
BEDBB - Combination of genes in order of ...<br><br />
DBEBB - Combination of genes in order of ...<br><br />
DBBBE - Combination of genes in order of ...<br><br />
<br />
== '''Lab Book and Calendar''' ==<br />
[[image: Alberta_butapipettips.jpg]][[image: Alberta_arrow.jpg]] [[image: Alberta_Labbook.jpg]] <br><br />
<br />
Butanerd Event Calendar can be found below:<br />
<br />
[http://www.ualberta.ca/~mjl3/UofAIgemProtocols.pdf The Lab Protocols]<br />
<br />
[[Alberta/Calender/July|July 2007]]<br />
<br />
[[Alberta/Calender/August|August 2007]]<br />
<br />
[[Alberta/Calender/September|September 2007]]<br />
<br />
[[Alberta/Calender/October|October 2007]]<br />
<br />
'''[http://butanerds.myfreeforum.org The Butanerd Online Forum]''' is up and running. Feel free to post and check for posts here. Make sure you register!<br />
<br />
<br />
== '''The Team''' ==<br />
<br />
<center>[[Image: Alberta_team_new.jpg]] </center><br />
<br><br />
<br />
Our team has a rich background in biology, biochemistry and engineering. To compliment our diversity we also have advisors who have a wealth of knowledge in research and applications of genetic engineering. For more information about the group, check out the '''[[Alberta/Members|University of Alberta's iGEM Team Members Page]]'''.<br />
<br />
Our University of Alberta Student Group Constitution can be found '''[[alberta/constitution|here]]'''<br />
<br />
== '''Edmonton''' ==<br />
<br />
Welcome to Edmonton!<br />
<br />
<center>[[Image: Alberta_edmonton.jpg]]</center><br />
<br><br />
<br />
For more on the city of Edmonton click '''[http://www.ualberta.ca/~mjl3/About.html here]'''.<br />
<br />
== '''Sponsors''' ==<br />
<br />
We would like to thank all of our sponsers for their gracious support and our advisors for invaluble advise.<br />
<br />
<br />
<b>Major Sponsors</b><br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors1.gif]]<br />
|}<br />
<br />
<br />
'''Sponsors'''<br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors2.gif]]<br />
|}<br />
<br />
'''Advice'''<br />
<br />
<br />
'''Andrew Hessel''' - ''Alberta Ingenuity Mentor''<br><br />
'''Dr. Jonathan Dennis''' - ''University of Alberta''<br><br />
'''Dr. Perrin Beatty''' - ''University of Alberta''<br><br />
'''Dr. David Bressler''' - ''University of Alberta''<br><br />
'''Dr. Charles Lucy''' - ''University of Alberta''<br><br />
'''Dr. Gregory Kiema''' - ''University of Alberta''<br><br />
'''Dr. Mark S. Peppler''' - ''University of Alberta''<br><br />
'''Dr. James Harynuk''' - ''University of Alberta''<br><br />
'''Dr. Federick West'''-''University of Alberta''<br><br />
'''Dr. Todd Lowary'''-''University of Alberta''<br><br />
'''Desiree Schell''' - ''University of Alberta CJSR''<br><br />
'''Dr. Jeff Fuller''' - ''University of Alberta/Capital Health''<br><br />
'''Dr. Julia Foght''' - ''University of Alberta''<br><br />
'''Dr. David Checkel''' - ''University of Alberta''<br><br />
'''Adrian Audiet''' - ''University of Alberta''<br><br />
'''Dr. Koch''' - ''University of Alberta''<br><br />
'''Dr. Donald Bryant''' - ''Pennsylvania State''<br><br />
'''Dr. Gaozhong Shen''' - ''Pennsylvania State''<br><br />
'''Amaya Garcia''' - ''Pennsylvania State''<br><br />
<br />
<br />
A list of experimental supplies we need can be found '''[[supplies|here]]'''<br />
<br />
== '''External Links''' ==<br />
<br />
[http://www.albertaingenuity.ca/ Alberta Ingenuity Fund]<br />
<br />
[http://www.ualberta.ca The University of Alberta Hompage]<br />
<br />
[http://www.mece.ualberta.ca/~ckoch/ Dr. Koch's Engine Lab]<br />
<br />
[http://www.systems-biology.org/cd Cell Designer Homepage]<br />
<br />
[http://www.biomodels.org Biomodels Home Page]</div>CelineYZhttp://2007.igem.org/wiki/index.php/AlbertaAlberta2007-10-26T20:49:24Z<p>CelineYZ: /* '''The Project: Plan B''' */</p>
<hr />
<div><center>[[Image: headbanner.jpg]] <br />
<br><br />
''The Offical Wiki of the 2007 University of Alberta iGEM Team'' <br><br />
''Edmonton, Alberta, Canada </center>''<br />
<br />
== '''Background: Biofuels'''==<br />
<br />
<br />
With growing concerns of global greenhouse gasses, the global energy market is in a continual push for the development of renewable energy sources. Specifically, two fuels have dominated the media: biodiesel and ethanol. However, both of these fuels have shortcomings in terms of being a viable fuel source.<br />
<br />
<br />
Biodiesel is a fuel produced from the vegetable oils of crops that can be used in an engine system very similar to a traditional diesel engine. However, vegetable oils in crops only make up a small portion of the biomass of the plants, ultimately producing low yields of fuel per acre of crops. As such, it is more economically sound to use these resources for food production.<br />
<br />
<br />
There has been huge attention to the use of ethanol in the typical auto cycle engine. In many places it is already being blended with gasoline to create a hybrid fuel source. However, running an engine on pure ethanol is beset by several major obstacles. Firstly, ethanol is miscible with water at any concentration, which creates long term storage corrosion issues. Secondly, ethanol engines must be designed to expect water vapor unlike their gasoline counterparts. Ethanol also has significantly different thermodynamic properties than gasoline such as a lower energy density and different vapor properties, which would reduce the economical advantage of using ethanol as a primary fuels source.<br />
<br />
<br />
We propose using a different fuel source to eventually replace gasoline, butanol. Butanol is a superior to ethanol as a replacement for petroleum gasoline. With a low vapor pressure, high energy density, and a gasoline-like octane rating, it can be blended into existing gasoline at much higher proportions than ethanol without compromising performance, mileage, organic pollution standards. Blending butanol with gasoline also prevents major modifications of the fuel-air ratio and modifications to the fuel system. Butanol also is immiscible with water at concentrations higher than 7%, alleviating storage concerns as well as offering the possiblity of phase separation which would realize huge cost savings in terms of production.<br />
<br />
<br />
More information on the summary of biofuels viability and the inspiration to our project can be found '''[[Alberta/background|here]]'''.<br />
<br />
<br />
The Butanerds wanted to further evaluate the use of butanol as a fuel in standard spark ignition engines. With the assistance of The University of Alberta's Engine Control Lab in Mechanical Engineering we were able to run a peak power test comparing butanol to iso-octane, a standard test measurement. Each fuel was burned at a stoichometric air to fuel ratio at 4 different angular speeds. The experimental Setup and results can be seen below.<br />
<br />
[[image: alberta_engine1.jpeg|thumb|left|275px| Engine Test Setup Front View]]<br />
[[image: alberta_engine2.jpeg|thumb|left|275px| Engine Test Setup Side View]]<br />
[[image: alberta_enginechart2.jpeg|thumb|left|275px| Peak Power vs. RPM Experimental Data]]<br />
<br><br />
<br />
== '''The Project: Plan B''' ==<br />
<br />
We propose the use of butanol as the leading biofuel for use in internal combustion engines. Specifically, we intend to genetically engineer ''Escherichia coli'' bacteria to convert biomasses into butanol for use as an energy source. This will be accomplished by introducing the genes responsible for butanol production from ''Clostridium acetobutylicum''(i.e. endogenous butanoate pathway) into ''E. coli''. Furthermore, we hope to increase ''E. coli''s tolerance to solvents such as butanol.<br />
<br />
<b> Objective I: Transforming genes in ''C. acetobutylicum'' butanoate pathway into ''E.coli''</b><br><br />
Genes encoding the enzymes in the ''C.acetobutylicum'' butanoate metabolism pathway are identified using KEGG database (KEGG number-ca00650: http://www.kegg.com/dbget-bin/www_bget?path:cac00650). Our cloning stragegy is to incorporate all the genes in a single operon with respective inducible promoter and ribosome binding site in a plasmid. Such construct enables easy transformation of multiple genes simultaneously into ''E.coli'' and allows the coordinated expression of genes within the operon. <br><br />
<br />
After receiving the commercially synthesized coding sequences of the genes of interest, the coding sequences are restricted with proper BioBrick prefices and suffices out of the original plasmid and cloned into B0034 (ribosome binding site). Double digest (Xba I and Spe I) as well as automated sequencing reactions are performed to verify the proper insertion of the coding sequences and the presence of ribosome binding site at the 5' end of each coding sequence. <br><br />
<br />
The operon, shown below, consists of all of the genes of the pathway (except thiolase). Note that we purposedly added a Histamine tag to the carboxy terminus of each gene product such that we could use Nickel-NTA column to purify the individual proteins and analyze protein expression and activity.<br />
[[image: alberta_butanol_Operon.jpeg|thumb|center|400px|The Butanol Operon]]<br />
<br />
Objective 2: Develop Butanol Tolerance with mutagenic and toxic compound Ethylnitrosourea ENU.<br><br />
The concept is to make butanol agar plates of various butanol concentrations and place and ENU disk in the centre. We expect there will be two kill zones. The first where bacteria die due to the toxicity of ENU at the centre of the plate. The second around the outer ring of the butanol plate where bacteria die due to butanol toxicity. In between these two zones we hope to see some cells that would be mutated in a benifical way to allow them to survive in the butanol.<br />
<br />
[[image: alberta_butanolplate2.jpeg|400px|thumb|center|Proposed Butanol-ENU Plate for Mutagenisis of ''E. coli'']]<br />
<br />
<br />
Concurrently, we are investigating the use of a photoautotrophic bacterium, ''Chlorobium tepidum'' that we will also introduce butanol producing genes to. ''Chlorobium tepidum'' is a green sulfur bacterium that is strictly anaerobic and uses sulfur compounds as terminal electron donors. ''Chlorobium tepidum'' is a moderately thermophillic bacterium, growing at 40 degrees Celsius (104 degrees Fahrenheit), and requires low light conditions for optimal growth. These bacteria grow well in a defined medium, utilizing the reverse/reductive tricarboxylic acid (TCA) cycle to build up carbohydrates from carbon dioxide. For a more comprehensive overview of ''Chlorobium tepidum'' go to [[http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html ''here'']]. Images below from http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html<br />
<br />
[[Image:spacer.jpeg|left]][[Image:Alberta_micrograph.gif|thumb|300px|left|Figure 1:''Chlorobium tepidum'' Transmission Micrograph Image]]<br />
[[Image:Alberta_gramstain.jpg|thumb|330px|left|Figure 2:''Chlorobium tepidum'' Standard Light Microscope Image]]<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br />
<br />
The advantage of manipulating this organism for butanol production is a matter of energy input and output. Rather than having to utilize vast amounts of food stocks, such as grains or sugars, a photoautotroph will fix carbon dioxide into the complex carbohydrates required for butanol production. Theoretically, if the only fuel on our planet was butanol, and it was produced in this manner, there would be little net carbon dioxide produced.<br />
<br />
[[image: alberta_carboncycle1.jpeg|thumb|300px|center| Propose Closed Carbon-butanol Cycle]]<br />
<br />
== '''Modeling Efforts''' ==<br />
<br />
Initially our plan to model the efficiency of the butanol production hinged on developing a complete model for the entire glucose pathway in an ''E. coli'' cell using Michaelis-Menten kinetics. After further review, this proved to be beyond the scope of our project's timeline due both to the large number of species and reactions present in the system (as well as their non-linear behavior) and the relative difficulty in finding experimental kinetic information for some of the reactions in our pathway. <br> <br />
<br />
<br />
Therefore, to obtain a rough "first-order" approximation for the behavior of the pathway, we considered two factors:<br />
<br />
1) Stoichiometric factors (both redox and carbon)<br><br />
2) Compared the relative production rates of the various end products of the glucose pathway in ''E. coli'' as found in previous experimentation to the production rates of butanol in ''C. acetobutylicum''.<br />
3) We have also a fuel E. coli Stoichometric Matrix, and intend to stoichiometrically model the system in order to optimize butanol production once the operon is in the system.<br />
<br />
<br />
From these factors we can obtain an indicator of how likely butanol will be produced.<br />
<br><br />
<br />
In order to further develop the system, we hope a full metabolic stochiometric model can be eventually realized for this project. This would allow optimization of the system in order to maximize the flux through the butanol pathway.<br />
<br />
<br />
== '''Project Timeline''' ==<br />
<br />
[[image: Alberta_gantt.jpeg|thumb|400px|center|[[Project Gantt Chart (click here to enlarge)]]]]<br />
<br />
The GANTT Chart Above details the schedule for the lab work that contributed to Plan B. Beginning in July and finishing with our final work in October, the schedule includes details on our different methods to compose an operon that would meet our objectives. In order to read the GANTT Chart for the Lab work of Plan B, please refer to the legend below.<br />
<br />
'''Legend: ''' <br><br />
Benny - <br><br />
Enny - <br><br />
Buddy - <br><br />
Betty - <br><br />
Deisel Blaze - <br><br />
J61003 - <br><br />
B0034 - <br><br />
I0500 - <br><br />
Thiolase - <br><br />
BBDBE - Combination of genes in order of ...<br><br />
BEDBB - Combination of genes in order of ...<br><br />
DBEBB - Combination of genes in order of ...<br><br />
DBBBE - Combination of genes in order of ...<br><br />
<br />
== '''Lab Book and Calendar''' ==<br />
[[image: Alberta_butapipettips.jpg]][[image: Alberta_arrow.jpg]] [[image: Alberta_Labbook.jpg]] <br><br />
<br />
Butanerd Event Calendar can be found below:<br />
<br />
[http://www.ualberta.ca/~mjl3/UofAIgemProtocols.pdf The Lab Protocols]<br />
<br />
[[Alberta/Calender/July|July 2007]]<br />
<br />
[[Alberta/Calender/August|August 2007]]<br />
<br />
[[Alberta/Calender/September|September 2007]]<br />
<br />
[[Alberta/Calender/October|October 2007]]<br />
<br />
'''[http://butanerds.myfreeforum.org The Butanerd Online Forum]''' is up and running. Feel free to post and check for posts here. Make sure you register!<br />
<br />
<br />
== '''The Team''' ==<br />
<br />
<center>[[Image: Alberta_team_new.jpg]] </center><br />
<br><br />
<br />
Our team has a rich background in biology, biochemistry and engineering. To compliment our diversity we also have advisors who have a wealth of knowledge in research and applications of genetic engineering. For more information about the group, check out the '''[[Alberta/Members|University of Alberta's iGEM Team Members Page]]'''.<br />
<br />
Our University of Alberta Student Group Constitution can be found '''[[alberta/constitution|here]]'''<br />
<br />
== '''Edmonton''' ==<br />
<br />
Welcome to Edmonton!<br />
<br />
<center>[[Image: Alberta_edmonton.jpg]]</center><br />
<br><br />
<br />
For more on the city of Edmonton click '''[http://www.ualberta.ca/~mjl3/About.html here]'''.<br />
<br />
== '''Sponsors''' ==<br />
<br />
We would like to thank all of our sponsers for their gracious support and our advisors for invaluble advise.<br />
<br />
<br />
<b>Major Sponsors</b><br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors1.gif]]<br />
|}<br />
<br />
<br />
'''Sponsors'''<br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors2.gif]]<br />
|}<br />
<br />
'''Advice'''<br />
<br />
<br />
'''Andrew Hessel''' - ''Alberta Ingenuity Mentor''<br><br />
'''Dr. Jonathan Dennis''' - ''University of Alberta''<br><br />
'''Dr. Perrin Beatty''' - ''University of Alberta''<br><br />
'''Dr. David Bressler''' - ''University of Alberta''<br><br />
'''Dr. Charles Lucy''' - ''University of Alberta''<br><br />
'''Dr. Gregory Kiema''' - ''University of Alberta''<br><br />
'''Dr. Mark S. Peppler''' - ''University of Alberta''<br><br />
'''Dr. James Harynuk''' - ''University of Alberta''<br><br />
'''Dr. Federick West'''-''University of Alberta''<br><br />
'''Dr. Todd Lowary'''-''University of Alberta''<br><br />
'''Desiree Schell''' - ''University of Alberta CJSR''<br><br />
'''Dr. Jeff Fuller''' - ''University of Alberta/Capital Health''<br><br />
'''Dr. Julia Foght''' - ''University of Alberta''<br><br />
'''Dr. David Checkel''' - ''University of Alberta''<br><br />
'''Adrian Audiet''' - ''University of Alberta''<br><br />
'''Dr. Koch''' - ''University of Alberta''<br><br />
'''Dr. Donald Bryant''' - ''Pennsylvania State''<br><br />
'''Dr. Gaozhong Shen''' - ''Pennsylvania State''<br><br />
'''Amaya Garcia''' - ''Pennsylvania State''<br><br />
<br />
<br />
A list of experimental supplies we need can be found '''[[supplies|here]]'''<br />
<br />
== '''External Links''' ==<br />
<br />
[http://www.albertaingenuity.ca/ Alberta Ingenuity Fund]<br />
<br />
[http://www.ualberta.ca The University of Alberta Hompage]<br />
<br />
[http://www.mece.ualberta.ca/~ckoch/ Dr. Koch's Engine Lab]<br />
<br />
[http://www.systems-biology.org/cd Cell Designer Homepage]<br />
<br />
[http://www.biomodels.org Biomodels Home Page]</div>CelineYZhttp://2007.igem.org/wiki/index.php/AlbertaAlberta2007-10-26T20:39:01Z<p>CelineYZ: /* '''The Project: Plan B''' */</p>
<hr />
<div><center>[[Image: headbanner.jpg]] <br />
<br><br />
''The Offical Wiki of the 2007 University of Alberta iGEM Team'' <br><br />
''Edmonton, Alberta, Canada </center>''<br />
<br />
== '''Background: Biofuels'''==<br />
<br />
<br />
With growing concerns of global greenhouse gasses, the global energy market is in a continual push for the development of renewable energy sources. Specifically, two fuels have dominated the media: biodiesel and ethanol. However, both of these fuels have shortcomings in terms of being a viable fuel source.<br />
<br />
<br />
Biodiesel is a fuel produced from the vegetable oils of crops that can be used in an engine system very similar to a traditional diesel engine. However, vegetable oils in crops only make up a small portion of the biomass of the plants, ultimately producing low yields of fuel per acre of crops. As such, it is more economically sound to use these resources for food production.<br />
<br />
<br />
There has been huge attention to the use of ethanol in the typical auto cycle engine. In many places it is already being blended with gasoline to create a hybrid fuel source. However, running an engine on pure ethanol is beset by several major obstacles. Firstly, ethanol is miscible with water at any concentration, which creates long term storage corrosion issues. Secondly, ethanol engines must be designed to expect water vapor unlike their gasoline counterparts. Ethanol also has significantly different thermodynamic properties than gasoline such as a lower energy density and different vapor properties, which would reduce the economical advantage of using ethanol as a primary fuels source.<br />
<br />
<br />
We propose using a different fuel source to eventually replace gasoline, butanol. Butanol is a superior to ethanol as a replacement for petroleum gasoline. With a low vapor pressure, high energy density, and a gasoline-like octane rating, it can be blended into existing gasoline at much higher proportions than ethanol without compromising performance, mileage, organic pollution standards. Blending butanol with gasoline also prevents major modifications of the fuel-air ratio and modifications to the fuel system. Butanol also is immiscible with water at concentrations higher than 7%, alleviating storage concerns as well as offering the possiblity of phase separation which would realize huge cost savings in terms of production.<br />
<br />
<br />
More information on the summary of biofuels viability and the inspiration to our project can be found '''[[Alberta/background|here]]'''.<br />
<br />
<br />
The Butanerds wanted to further evaluate the use of butanol as a fuel in standard spark ignition engines. With the assistance of The University of Alberta's Engine Control Lab in Mechanical Engineering we were able to run a peak power test comparing butanol to iso-octane, a standard test measurement. Each fuel was burned at a stoichometric air to fuel ratio at 4 different angular speeds. The experimental Setup and results can be seen below.<br />
<br />
[[image: alberta_engine1.jpeg|thumb|left|275px| Engine Test Setup Front View]]<br />
[[image: alberta_engine2.jpeg|thumb|left|275px| Engine Test Setup Side View]]<br />
[[image: alberta_enginechart2.jpeg|thumb|left|275px| Peak Power vs. RPM Experimental Data]]<br />
<br><br />
<br />
== '''The Project: Plan B''' ==<br />
<br />
We propose the use of butanol as the leading biofuel for use in internal combustion engines. Specifically, we intend to genetically engineer ''Escherichia coli'' bacteria to convert biomasses into butanol for use as an energy source. This will be accomplished by introducing the genes responsible for butanol production from ''Clostridium acetobutylicum''(i.e. endogenous butanoate pathway) into ''E. coli''. Furthermore, we hope to increase ''E. coli''s'' tolerance to solvents such as butanol.<br />
<br />
Objective I: Transforming genes in ''C. acetobutylicum'' butanoate pathway into ''E.coli''<br><br />
Genes encoding the enzymes in the ''C.acetobutylicum'' butanoate metabolism pathway were identified using KEGG database (KEGG number-ca00650: http://www.kegg.com/dbget-bin/www_bget?path:cac00650). Our cloning stragegy was to incorporate all the genes in a single operon with respective inducible promoter and ribosome binding site in a plasmid. Such construct enables easy transformation of multiple genes simultaneously into ''E.coli'' and allows the coordinated expression of genes within the operon. <br><br />
<br />
The operon, shown below, consists of all of the genes of the pathway (except thiolase) and His tags for ease of protien purification.<br />
<br />
[[image: alberta_butanol_Operon.jpeg|thumb|center|400px|The Butanol Operon]]<br />
<br />
To develop butanol tolerance with the aid of a mutagen Ethylnitrosourea ENU. The concept is to make butanol agar plates of various butanol concentrations and place and ENU disk in the centre. We expect there will be to kill zones. The first where bacteria die due to the toxicity of ENU at the centre of the plate. The second around the ring of the butanol plate where bacteria die due to butanol toxicity. In between these two zones we hope to see some cell that were mutated in a benifical way to allow them to survive in the butanol.<br />
<br />
[[image: alberta_butanolplate2.jpeg|400px|thumb|center|Proposed Butanol-ENU Plate for Mutagenisis of ''E. coli'']]<br />
<br />
<br />
Concurrently, we are investigating the use of a photoautotrophic bacterium, ''Chlorobium tepidum'' that we will also introduce butanol producing genes to. ''Chlorobium tepidum'' is a green sulfur bacterium that is strictly anaerobic and uses sulfur compounds as terminal electron donors. ''Chlorobium tepidum'' is a moderately thermophillic bacterium, growing at 40 degrees Celsius (104 degrees Fahrenheit), and requires low light conditions for optimal growth. These bacteria grow well in a defined medium, utilizing the reverse/reductive tricarboxylic acid (TCA) cycle to build up carbohydrates from carbon dioxide. For a more comprehensive overview of ''Chlorobium tepidum'' go to [[http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html ''here'']]. Images below from http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html<br />
<br />
[[Image:spacer.jpeg|left]][[Image:Alberta_micrograph.gif|thumb|300px|left|Figure 1:''Chlorobium tepidum'' Transmission Micrograph Image]]<br />
[[Image:Alberta_gramstain.jpg|thumb|330px|left|Figure 2:''Chlorobium tepidum'' Standard Light Microscope Image]]<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br />
<br />
The advantage of manipulating this organism for butanol production is a matter of energy input and output. Rather than having to utilize vast amounts of food stocks, such as grains or sugars, a photoautotroph will fix carbon dioxide into the complex carbohydrates required for butanol production. Theoretically, if the only fuel on our planet was butanol, and it was produced in this manner, there would be little net carbon dioxide produced.<br />
<br />
[[image: alberta_carboncycle1.jpeg|thumb|300px|center| Propose Closed Carbon-butanol Cycle]]<br />
<br />
== '''Modeling Efforts''' ==<br />
<br />
Initially our plan to model the efficiency of the butanol production hinged on developing a complete model for the entire glucose pathway in an ''E. coli'' cell using Michaelis-Menten kinetics. After further review, this proved to be beyond the scope of our project's timeline due both to the large number of species and reactions present in the system (as well as their non-linear behavior) and the relative difficulty in finding experimental kinetic information for some of the reactions in our pathway. <br> <br />
<br />
<br />
Therefore, to obtain a rough "first-order" approximation for the behavior of the pathway, we considered two factors:<br />
<br />
1) Stoichiometric factors (both redox and carbon)<br><br />
2) Compared the relative production rates of the various end products of the glucose pathway in ''E. coli'' as found in previous experimentation to the production rates of butanol in ''C. acetobutylicum''.<br />
3) We have also a fuel E. coli Stoichometric Matrix, and intend to stoichiometrically model the system in order to optimize butanol production once the operon is in the system.<br />
<br />
<br />
From these factors we can obtain an indicator of how likely butanol will be produced.<br />
<br><br />
<br />
In order to further develop the system, we hope a full metabolic stochiometric model can be eventually realized for this project. This would allow optimization of the system in order to maximize the flux through the butanol pathway.<br />
<br />
<br />
== '''Project Timeline''' ==<br />
<br />
[[image: Alberta_gantt.jpeg|thumb|400px|center|[[Project Gantt Chart (click here to enlarge)]]]]<br />
<br />
The GANTT Chart Above details the schedule for the lab work that contributed to Plan B. Beginning in July and finishing with our final work in October, the schedule includes details on our different methods to compose an operon that would meet our objectives. In order to read the GANTT Chart for the Lab work of Plan B, please refer to the legend below.<br />
<br />
'''Legend: ''' <br><br />
Benny - <br><br />
Enny - <br><br />
Buddy - <br><br />
Betty - <br><br />
Deisel Blaze - <br><br />
J61003 - <br><br />
B0034 - <br><br />
I0500 - <br><br />
Thiolase - <br><br />
BBDBE - Combination of genes in order of ...<br><br />
BEDBB - Combination of genes in order of ...<br><br />
DBEBB - Combination of genes in order of ...<br><br />
DBBBE - Combination of genes in order of ...<br><br />
<br />
== '''Lab Book and Calendar''' ==<br />
[[image: Alberta_butapipettips.jpg]][[image: Alberta_arrow.jpg]] [[image: Alberta_Labbook.jpg]] <br><br />
<br />
Butanerd Event Calendar can be found below:<br />
<br />
[http://www.ualberta.ca/~mjl3/UofAIgemProtocols.pdf The Lab Protocols]<br />
<br />
[[Alberta/Calender/July|July 2007]]<br />
<br />
[[Alberta/Calender/August|August 2007]]<br />
<br />
[[Alberta/Calender/September|September 2007]]<br />
<br />
[[Alberta/Calender/October|October 2007]]<br />
<br />
'''[http://butanerds.myfreeforum.org The Butanerd Online Forum]''' is up and running. Feel free to post and check for posts here. Make sure you register!<br />
<br />
<br />
== '''The Team''' ==<br />
<br />
<center>[[Image: Alberta_team_new.jpg]] </center><br />
<br><br />
<br />
Our team has a rich background in biology, biochemistry and engineering. To compliment our diversity we also have advisors who have a wealth of knowledge in research and applications of genetic engineering. For more information about the group, check out the '''[[Alberta/Members|University of Alberta's iGEM Team Members Page]]'''.<br />
<br />
Our University of Alberta Student Group Constitution can be found '''[[alberta/constitution|here]]'''<br />
<br />
== '''Edmonton''' ==<br />
<br />
Welcome to Edmonton!<br />
<br />
<center>[[Image: Alberta_edmonton.jpg]]</center><br />
<br><br />
<br />
For more on the city of Edmonton click '''[http://www.ualberta.ca/~mjl3/About.html here]'''.<br />
<br />
== '''Sponsors''' ==<br />
<br />
We would like to thank all of our sponsers for their gracious support and our advisors for invaluble advise.<br />
<br />
<br />
<b>Major Sponsors</b><br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors1.gif]]<br />
|}<br />
<br />
<br />
'''Sponsors'''<br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors2.gif]]<br />
|}<br />
<br />
'''Advice'''<br />
<br />
<br />
'''Andrew Hessel''' - ''Alberta Ingenuity Mentor''<br><br />
'''Dr. Jonathan Dennis''' - ''University of Alberta''<br><br />
'''Dr. Perrin Beatty''' - ''University of Alberta''<br><br />
'''Dr. David Bressler''' - ''University of Alberta''<br><br />
'''Dr. Charles Lucy''' - ''University of Alberta''<br><br />
'''Dr. Gregory Kiema''' - ''University of Alberta''<br><br />
'''Dr. Mark S. Peppler''' - ''University of Alberta''<br><br />
'''Dr. James Harynuk''' - ''University of Alberta''<br><br />
'''Dr. Federick West'''-''University of Alberta''<br><br />
'''Dr. Todd Lowary'''-''University of Alberta''<br><br />
'''Desiree Schell''' - ''University of Alberta CJSR''<br><br />
'''Dr. Jeff Fuller''' - ''University of Alberta/Capital Health''<br><br />
'''Dr. Julia Foght''' - ''University of Alberta''<br><br />
'''Dr. David Checkel''' - ''University of Alberta''<br><br />
'''Adrian Audiet''' - ''University of Alberta''<br><br />
'''Dr. Koch''' - ''University of Alberta''<br><br />
'''Dr. Donald Bryant''' - ''Pennsylvania State''<br><br />
'''Dr. Gaozhong Shen''' - ''Pennsylvania State''<br><br />
'''Amaya Garcia''' - ''Pennsylvania State''<br><br />
<br />
<br />
A list of experimental supplies we need can be found '''[[supplies|here]]'''<br />
<br />
== '''External Links''' ==<br />
<br />
[http://www.albertaingenuity.ca/ Alberta Ingenuity Fund]<br />
<br />
[http://www.ualberta.ca The University of Alberta Hompage]<br />
<br />
[http://www.mece.ualberta.ca/~ckoch/ Dr. Koch's Engine Lab]<br />
<br />
[http://www.systems-biology.org/cd Cell Designer Homepage]<br />
<br />
[http://www.biomodels.org Biomodels Home Page]</div>CelineYZhttp://2007.igem.org/wiki/index.php/AlbertaAlberta2007-10-26T20:25:04Z<p>CelineYZ: /* '''The Project: Plan B''' */</p>
<hr />
<div><center>[[Image: headbanner.jpg]] <br />
<br><br />
''The Offical Wiki of the 2007 University of Alberta iGEM Team'' <br><br />
''Edmonton, Alberta, Canada </center>''<br />
<br />
== '''Background: Biofuels'''==<br />
<br />
<br />
With growing concerns of global greenhouse gasses, the global energy market is in a continual push for the development of renewable energy sources. Specifically, two fuels have dominated the media: biodiesel and ethanol. However, both of these fuels have shortcomings in terms of being a viable fuel source.<br />
<br />
<br />
Biodiesel is a fuel produced from the vegetable oils of crops that can be used in an engine system very similar to a traditional diesel engine. However, vegetable oils in crops only make up a small portion of the biomass of the plants, ultimately producing low yields of fuel per acre of crops. As such, it is more economically sound to use these resources for food production.<br />
<br />
<br />
There has been huge attention to the use of ethanol in the typical auto cycle engine. In many places it is already being blended with gasoline to create a hybrid fuel source. However, running an engine on pure ethanol is beset by several major obstacles. Firstly, ethanol is miscible with water at any concentration, which creates long term storage corrosion issues. Secondly, ethanol engines must be designed to expect water vapor unlike their gasoline counterparts. Ethanol also has significantly different thermodynamic properties than gasoline such as a lower energy density and different vapor properties, which would reduce the economical advantage of using ethanol as a primary fuels source.<br />
<br />
<br />
We propose using a different fuel source to eventually replace gasoline, butanol. Butanol is a superior to ethanol as a replacement for petroleum gasoline. With a low vapor pressure, high energy density, and a gasoline-like octane rating, it can be blended into existing gasoline at much higher proportions than ethanol without compromising performance, mileage, organic pollution standards. Blending butanol with gasoline also prevents major modifications of the fuel-air ratio and modifications to the fuel system. Butanol also is immiscible with water at concentrations higher than 7%, alleviating storage concerns as well as offering the possiblity of phase separation which would realize huge cost savings in terms of production.<br />
<br />
<br />
More information on the summary of biofuels viability and the inspiration to our project can be found '''[[Alberta/background|here]]'''.<br />
<br />
<br />
The Butanerds wanted to further evaluate the use of butanol as a fuel in standard spark ignition engines. With the assistance of The University of Alberta's Engine Control Lab in Mechanical Engineering we were able to run a peak power test comparing butanol to iso-octane, a standard test measurement. Each fuel was burned at a stoichometric air to fuel ratio at 4 different angular speeds. The experimental Setup and results can be seen below.<br />
<br />
[[image: alberta_engine1.jpeg|thumb|left|275px| Engine Test Setup Front View]]<br />
[[image: alberta_engine2.jpeg|thumb|left|275px| Engine Test Setup Side View]]<br />
[[image: alberta_enginechart2.jpeg|thumb|left|275px| Peak Power vs. RPM Experimental Data]]<br />
<br><br />
<br />
== '''The Project: Plan B''' ==<br />
<br />
We propose the use of butanol as the leading biofuel for use in internal combustion engines. Specifically, we intend to genetically engineer ''Escherichia coli'' bacteria to convert biomasses into butanol for use as an energy source. This will be accomplished by introducing the genes responsible for butanol production from ''''Clostridium acetobutylicum''''(i.e. endogenous butanoate pathway) into ''''E. coli''''. Furthermore, we hope to increase ''''E. coli'''s'' tolerance to solvents such as butanol.<br />
<br />
We have choosen to put all of the genes in a single operon to make the stucture easy to transform as well as so that all genes in the operon have similar expression levels. The operon, shown below, consists of all of the genes of the pathway and his tags for ease of protien purification.<br />
<br />
[[image: alberta_butanol_Operon.jpeg|thumb|center|400px|The Butanol Operon]]<br />
<br />
To develop butanol tolerance with the aid of a mutagen Ethylnitrosourea ENU. The concept is to make butanol agar plates of various butanol concentrations and place and ENU disk in the centre. We expect there will be to kill zones. The first where bacteria die due to the toxicity of ENU at the centre of the plate. The second around the ring of the butanol plate where bacteria die due to butanol toxicity. In between these two zones we hope to see some cell that were mutated in a benifical way to allow them to survive in the butanol.<br />
<br />
[[image: alberta_butanolplate2.jpeg|400px|thumb|center|Proposed Butanol-ENU Plate for Mutagenisis of ''E. coli'']]<br />
<br />
<br />
Concurrently, we are investigating the use of a photoautotrophic bacterium, ''Chlorobium tepidum'' that we will also introduce butanol producing genes to. ''Chlorobium tepidum'' is a green sulfur bacterium that is strictly anaerobic and uses sulfur compounds as terminal electron donors. ''Chlorobium tepidum'' is a moderately thermophillic bacterium, growing at 40 degrees Celsius (104 degrees Fahrenheit), and requires low light conditions for optimal growth. These bacteria grow well in a defined medium, utilizing the reverse/reductive tricarboxylic acid (TCA) cycle to build up carbohydrates from carbon dioxide. For a more comprehensive overview of ''Chlorobium tepidum'' go to [[http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html ''here'']]. Images below from http://www.bmb.psu.edu/faculty/bryant/lab/chlorobiumtepidum/index.html<br />
<br />
[[Image:spacer.jpeg|left]][[Image:Alberta_micrograph.gif|thumb|300px|left|Figure 1:''Chlorobium tepidum'' Transmission Micrograph Image]]<br />
[[Image:Alberta_gramstain.jpg|thumb|330px|left|Figure 2:''Chlorobium tepidum'' Standard Light Microscope Image]]<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br />
<br />
<br />
The advantage of manipulating this organism for butanol production is a matter of energy input and output. Rather than having to utilize vast amounts of food stocks, such as grains or sugars, a photoautotroph will fix carbon dioxide into the complex carbohydrates required for butanol production. Theoretically, if the only fuel on our planet was butanol, and it was produced in this manner, there would be little net carbon dioxide produced.<br />
<br />
[[image: alberta_carboncycle1.jpeg|thumb|300px|center| Propose Closed Carbon-butanol Cycle]]<br />
<br />
== '''Modeling Efforts''' ==<br />
<br />
Initially our plan to model the efficiency of the butanol production hinged on developing a complete model for the entire glucose pathway in an ''E. coli'' cell using Michaelis-Menten kinetics. After further review, this proved to be beyond the scope of our project's timeline due both to the large number of species and reactions present in the system (as well as their non-linear behavior) and the relative difficulty in finding experimental kinetic information for some of the reactions in our pathway. <br> <br />
<br />
<br />
Therefore, to obtain a rough "first-order" approximation for the behavior of the pathway, we considered two factors:<br />
<br />
1) Stoichiometric factors (both redox and carbon)<br><br />
2) Compared the relative production rates of the various end products of the glucose pathway in ''E. coli'' as found in previous experimentation to the production rates of butanol in ''C. acetobutylicum''.<br />
3) We have also a fuel E. coli Stoichometric Matrix, and intend to stoichiometrically model the system in order to optimize butanol production once the operon is in the system.<br />
<br />
<br />
From these factors we can obtain an indicator of how likely butanol will be produced.<br />
<br><br />
<br />
In order to further develop the system, we hope a full metabolic stochiometric model can be eventually realized for this project. This would allow optimization of the system in order to maximize the flux through the butanol pathway.<br />
<br />
<br />
== '''Project Timeline''' ==<br />
<br />
[[image: Alberta_gantt.jpeg|thumb|400px|center|[[Project Gantt Chart (click here to enlarge)]]]]<br />
<br />
The GANTT Chart Above details the schedule for the lab work that contributed to Plan B. Beginning in July and finishing with our final work in October, the schedule includes details on our different methods to compose an operon that would meet our objectives. In order to read the GANTT Chart for the Lab work of Plan B, please refer to the legend below.<br />
<br />
'''Legend: ''' <br><br />
Benny - <br><br />
Enny - <br><br />
Buddy - <br><br />
Betty - <br><br />
Deisel Blaze - <br><br />
J61003 - <br><br />
B0034 - <br><br />
I0500 - <br><br />
Thiolase - <br><br />
BBDBE - Combination of genes in order of ...<br><br />
BEDBB - Combination of genes in order of ...<br><br />
DBEBB - Combination of genes in order of ...<br><br />
DBBBE - Combination of genes in order of ...<br><br />
<br />
== '''Lab Book and Calendar''' ==<br />
[[image: Alberta_butapipettips.jpg]][[image: Alberta_arrow.jpg]] [[image: Alberta_Labbook.jpg]] <br><br />
<br />
Butanerd Event Calendar can be found below:<br />
<br />
[http://www.ualberta.ca/~mjl3/UofAIgemProtocols.pdf The Lab Protocols]<br />
<br />
[[Alberta/Calender/July|July 2007]]<br />
<br />
[[Alberta/Calender/August|August 2007]]<br />
<br />
[[Alberta/Calender/September|September 2007]]<br />
<br />
[[Alberta/Calender/October|October 2007]]<br />
<br />
'''[http://butanerds.myfreeforum.org The Butanerd Online Forum]''' is up and running. Feel free to post and check for posts here. Make sure you register!<br />
<br />
<br />
== '''The Team''' ==<br />
<br />
<center>[[Image: Alberta_team_new.jpg]] </center><br />
<br><br />
<br />
Our team has a rich background in biology, biochemistry and engineering. To compliment our diversity we also have advisors who have a wealth of knowledge in research and applications of genetic engineering. For more information about the group, check out the '''[[Alberta/Members|University of Alberta's iGEM Team Members Page]]'''.<br />
<br />
Our University of Alberta Student Group Constitution can be found '''[[alberta/constitution|here]]'''<br />
<br />
== '''Edmonton''' ==<br />
<br />
Welcome to Edmonton!<br />
<br />
<center>[[Image: Alberta_edmonton.jpg]]</center><br />
<br><br />
<br />
For more on the city of Edmonton click '''[http://www.ualberta.ca/~mjl3/About.html here]'''.<br />
<br />
== '''Sponsors''' ==<br />
<br />
We would like to thank all of our sponsers for their gracious support and our advisors for invaluble advise.<br />
<br />
<br />
<b>Major Sponsors</b><br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors1.gif]]<br />
|}<br />
<br />
<br />
'''Sponsors'''<br />
<br />
{| align="center" style="color:white;"<br />
|[[Image: Alberta_sponsors2.gif]]<br />
|}<br />
<br />
'''Advice'''<br />
<br />
<br />
'''Andrew Hessel''' - ''Alberta Ingenuity Mentor''<br><br />
'''Dr. Jonathan Dennis''' - ''University of Alberta''<br><br />
'''Dr. Perrin Beatty''' - ''University of Alberta''<br><br />
'''Dr. David Bressler''' - ''University of Alberta''<br><br />
'''Dr. Charles Lucy''' - ''University of Alberta''<br><br />
'''Dr. Gregory Kiema''' - ''University of Alberta''<br><br />
'''Dr. Mark S. Peppler''' - ''University of Alberta''<br><br />
'''Dr. James Harynuk''' - ''University of Alberta''<br><br />
'''Dr. Federick West'''-''University of Alberta''<br><br />
'''Dr. Todd Lowary'''-''University of Alberta''<br><br />
'''Desiree Schell''' - ''University of Alberta CJSR''<br><br />
'''Dr. Jeff Fuller''' - ''University of Alberta/Capital Health''<br><br />
'''Dr. Julia Foght''' - ''University of Alberta''<br><br />
'''Dr. David Checkel''' - ''University of Alberta''<br><br />
'''Adrian Audiet''' - ''University of Alberta''<br><br />
'''Dr. Koch''' - ''University of Alberta''<br><br />
'''Dr. Donald Bryant''' - ''Pennsylvania State''<br><br />
'''Dr. Gaozhong Shen''' - ''Pennsylvania State''<br><br />
'''Amaya Garcia''' - ''Pennsylvania State''<br><br />
<br />
<br />
A list of experimental supplies we need can be found '''[[supplies|here]]'''<br />
<br />
== '''External Links''' ==<br />
<br />
[http://www.albertaingenuity.ca/ Alberta Ingenuity Fund]<br />
<br />
[http://www.ualberta.ca The University of Alberta Hompage]<br />
<br />
[http://www.mece.ualberta.ca/~ckoch/ Dr. Koch's Engine Lab]<br />
<br />
[http://www.systems-biology.org/cd Cell Designer Homepage]<br />
<br />
[http://www.biomodels.org Biomodels Home Page]</div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-05T21:36:41Z<p>CelineYZ: /* October 5 */</p>
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font-family: Verdana, Arial, Helvetica, sans-serif;<br />
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<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
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<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
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<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
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<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
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<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
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</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 8 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 9 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 10 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 11 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 12 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 13 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 14 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 15 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 16 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 17 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 18 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 19 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 20 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 21 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 22 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 23 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 24 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 25 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 26 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 27 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 28 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 29 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
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== October 30 ==<br />
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[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-05T03:00:45Z<p>CelineYZ: /* October 5 */</p>
<hr />
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<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
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<td>M</td><br />
<td>Tu</td><br />
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</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
<br />
<br />
CZ - night (should be able to come)<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-03T20:18:37Z<p>CelineYZ: /* October 5 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
CZ - 7:00pm<br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
CZ - night (to be confirmed)<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-09-30T20:26:03Z<p>CelineYZ: /* October 5 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
CZ - 7:00pm<br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
CZ - 2:30pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-09-30T20:25:51Z<p>CelineYZ: /* October 5 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
CZ - 7:00pm<br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs<br />
CZ - 2:30pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-09-30T20:24:16Z<p>CelineYZ: /* October 3 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
CZ - 7:00pm<br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-09-30T20:23:39Z<p>CelineYZ: /* October 1 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
CZ - 2:30pm <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-09-29T04:57:55Z<p>CelineYZ: /* October 3 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
<br />
CZ - 2:30pm<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
CZ - 2:30pm<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-09-29T04:57:33Z<p>CelineYZ: /* October 1 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
<br />
CZ - 2:30pm<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-24T02:31:59Z<p>CelineYZ: /* September 29 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
Poster Meeting @ 11:00am<br />
Meet in sub By the Subway--JG<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM -JG @ 8000hrs <br><br />
MC @ 845 - going to stop by dean of students' to pick up swag first.<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
930-1230 NK<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
JG & MC - 8:00AM <br><br />
NK 930-1230 <br><br />
CZ 2:30pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
CZ 2:00 pm<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-24T02:31:32Z<p>CelineYZ: /* September 28 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
Poster Meeting @ 11:00am<br />
Meet in sub By the Subway--JG<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM -JG @ 8000hrs <br><br />
MC @ 845 - going to stop by dean of students' to pick up swag first.<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
930-1230 NK<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
JG & MC - 8:00AM <br><br />
NK 930-1230 <br><br />
CZ 2:30pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>CelineYZhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-17T18:12:44Z<p>CelineYZ: /* September 19 */</p>
<hr />
<div>=='''September'''==<br />
<br />
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<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
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<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
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<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br />
<br />
11-2:<br />
AL,ML<br />
<br />
2-5:<br />
VH,NK<br />
<br />
5-8:<br />
JP<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br />
<br />
Night- ED JP<br />
<br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH (I will be in at about 1 or 1:30PM, if there is something written down for me to do I can work at it by myself, otherwise if someone wants to come in and get me started on something that would be nice.)<br />
<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Sorry I have meeting at 6pm which should last no longer than 2 hours, so I will come at 8pm the latest.<br><br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Hey guys, i wont be able to make it till 930 AM-NK<br />
<br />
PM - VH, JG<br />
<br />
Meeting 7:00<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL<br />
<br />
PM - VH, CZ<br />
<br />
night - JP<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br />
<br />
11-2 - JG, ML<br />
<br />
2-5 - NG, CZ<br />
<br />
5-8 - NK, I have it covered.<br />
<br />
Could not do transformation ligations because could not find competent cells. The ligations are in the -20 freezer with the green tape saying "ligations, sept 15". -NK<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br />
<br />
11-2 VH, CZ<br />
<br />
2-5 MC, JP<br />
<br />
5-8 - ML, NG<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs), ML (~10:30-1:00)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~12:30 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG- (@5PM)<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>CelineYZ