T7-RBS I716306

From 2007.igem.org

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Biobrickificreation of T7 Promoters I716105
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Biobrickificreation of T7 Promoters I716105 <br>
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PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A)<br>
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PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A)
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Sub into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L)<br>
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Sub into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L)
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Product A is pBca9145-I716105-PT7-wt <br>
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Product A is pBca9145-I716105-PT7-wt
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dt008-PT7-wt T7 Promoter (Wild Type)    GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa <br>
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Sub into pJD507_pSB1A2C (EcoRI, BglII 2071bp, 9bp L) <br>
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dt008-PT7-wt T7 Promoter (Wild Type)    GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa
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Sub into pJD507_pSB1A2C (EcoRI, BglII 2071bp, 9bp L)
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Produst is I716306
Produst is I716306

Latest revision as of 21:14, 30 July 2007

Biobrickificreation of T7 Promoters I716105
PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A)
Sub into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L)
Product A is pBca9145-I716105-PT7-wt
dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa
Sub into pJD507_pSB1A2C (EcoRI, BglII 2071bp, 9bp L)
Produst is I716306