TOPO Cloning
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The pTrcHis TOPO TA Expression Kit provides an efficient cloning strategy for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for expression in E. coli. No ligase, post-PCR procedures, or PCR primers containing specific sequences are required. Once the PCR product of interest is TOPO Cloned into pTrcHis-TOPO, the construct is transformed into E. coli and expression is induced with isopropyl β-thiogalactoside (IPTG). | The pTrcHis TOPO TA Expression Kit provides an efficient cloning strategy for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for expression in E. coli. No ligase, post-PCR procedures, or PCR primers containing specific sequences are required. Once the PCR product of interest is TOPO Cloned into pTrcHis-TOPO, the construct is transformed into E. coli and expression is induced with isopropyl β-thiogalactoside (IPTG). | ||
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Revision as of 17:25, 6 July 2007
TOPO cloning TOPO overview: The pTrcHis TOPO TA Expression Kit provides an efficient cloning strategy for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for expression in E. coli. No ligase, post-PCR procedures, or PCR primers containing specific sequences are required. Once the PCR product of interest is TOPO Cloned into pTrcHis-TOPO, the construct is transformed into E. coli and expression is induced with isopropyl β-thiogalactoside (IPTG).