TOPO Cloning
From 2007.igem.org
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TOPO overview: | TOPO overview: | ||
The pTrcHis TOPO TA Expression Kit provides an efficient cloning strategy for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for expression in E. coli. No ligase, post-PCR procedures, or PCR primers containing specific sequences are required. Once the PCR product of interest is TOPO Cloned into pTrcHis-TOPO, the construct is transformed into E. coli and expression is induced with isopropyl β-thiogalactoside (IPTG). | The pTrcHis TOPO TA Expression Kit provides an efficient cloning strategy for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for expression in E. coli. No ligase, post-PCR procedures, or PCR primers containing specific sequences are required. Once the PCR product of interest is TOPO Cloned into pTrcHis-TOPO, the construct is transformed into E. coli and expression is induced with isopropyl β-thiogalactoside (IPTG). | ||
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Revision as of 17:25, 6 July 2007
TOPO cloning
TOPO overview:
The pTrcHis TOPO TA Expression Kit provides an efficient cloning strategy for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for expression in E. coli. No ligase, post-PCR procedures, or PCR primers containing specific sequences are required. Once the PCR product of interest is TOPO Cloned into pTrcHis-TOPO, the construct is transformed into E. coli and expression is induced with isopropyl β-thiogalactoside (IPTG).
