ThpC

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PCR ThpC F/R on ThpC (421bp, BglII/XhoI)
Sub into pBca9145-Bca1144 (BgIII/XhoI, 2054+910 L)
Product is pBca9145-BBa I716333



ThpCF Forward BglII site anneals to ThpC ctagtAGATCTgtgatcgtccgatcgttcag

ThpAR Reverse XhoI site anneals to ThpC gtctactcgagtcaggcctcctcggtcagca