ThpD

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PCR ThpD F/R on ThpD (916bp, BglII/XhoI)
Sub into pBca9145-Bca1144 (BglII/XhoI, 2054+910 L)
Product is pBca9145-BBa I716334

ThpDF Forwar BglII site anneals to ThpD cggatAGATCTatgaccaccgaagtacgcgc

ThpDR Reverse XhoI site anneals to ThpD gtcagctcgagctacttcaccggggtgaagt

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