Tianjin/DIODE

From 2007.igem.org

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(Design)
(Model)
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==Model==
==Model==
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We make a mathematical model to simulate the behavior of the system.The model is base on ODEs.With the help of this model,we shows the ideal performance of each immobilized cells ,especially the amplifier cell, under different cell number and flux conditions.We also simulate the whole system at positive and negative and find out the difference of each cell of at positive and  negative as well as the situation the diode was broken down.<br>
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The model can help us with design and experiment.
<font size="3" color="#0000CC" bgcolor="#FF9933">1. </font>[[Tianjin/DIODE/Model0|<font size="3" color="#0000CC">Model design and construction</font>]]
<font size="3" color="#0000CC" bgcolor="#FF9933">1. </font>[[Tianjin/DIODE/Model0|<font size="3" color="#0000CC">Model design and construction</font>]]

Revision as of 07:17, 26 October 2007

Design

Brief Introduction of Bio-diode

The diode, one of the most significant parts in the electric integrate circuit and electronic equipment, functions as allowing the current get through when the diode is positive-biased, and cut off while it is negative-biased. When the negative voltage increases to a threshold, the diode tends to be negatively broken down. In this project, the molecular signal AHL is considered as the current of electric circuit.Three types of cells: Generator cellsAmplifier cells and Block cells are immobilized into small beads with a diameter of 0.5 millimeter, then different beads are settled into particular parts of our specially designed equipment sequentially and respectively.
The liquid culture flow through the generator cells and carry the AHL signal into the amplifier cells which are activated to make more AHL signals.
Then the concentration of AHL accumulates to such a level that block cells could not eliminate them completely, and the rest could be detected by Reporter cells, a condition called ‘posivive-biased pass’.

Diodedh.gif OK! Happy.gif

On the contrary, if the positions of amplifier cells and block cells in the equipment are reversed, the AHL produced by generator cell would be degraded by block cells at the very beginning resulting in no amplification of the signal. Thus, hardly AHL could be detected in the outflow culture, a condition called ‘negative-biased block’.

Diodeblock.gif Blocked!Sad.gif



Breakdown.gif Broken down!Cry.gif



Model

We make a mathematical model to simulate the behavior of the system.The model is base on ODEs.With the help of this model,we shows the ideal performance of each immobilized cells ,especially the amplifier cell, under different cell number and flux conditions.We also simulate the whole system at positive and negative and find out the difference of each cell of at positive and negative as well as the situation the diode was broken down.
The model can help us with design and experiment. 1. Model design and construction


2. Model result

2.1 Model of Generator Cells
TJU4L1.jpgTJU4L2.jpgTJU4L.jpgTJU8P1.jpgTJU8P2.jpg
2.2 Model of Block Cells
TJU4j.jpgTJU4j2.jpg
2.3 Model of Amplifier Cells
TJU8P1.jpgTJU8P2.jpg
2.4 Model of whole system
TJUI.jpgTJUI1.jpgTJUI2.jpgTJUIJ.jpgTJUIJP.jpgTJUzf.jpg

Experiment

1.Datas and Graphs of Detector cells

2.Function of immobilized cells

3.Assembly of equipment