Tianjin/DIODE/Experiment1/Function of Immobilized cells2

From 2007.igem.org

(Difference between revisions)
Line 2: Line 2:
<br>
<br>
[[Image:TJUQ1.jpg|400px]][[Image:TJUQ2.jpg|400px]]
[[Image:TJUQ1.jpg|400px]][[Image:TJUQ2.jpg|400px]]
 +
<br><br><br><br>
 +
Figure 2: One of our strategy is to cut down the functional part from original plasmid and transfer into a plasmid with a copy number less than 10 without the addition of IPTG (pSB2K3). However,
 +
 +
[[Image:TJUQ3.jpg|400px]]

Revision as of 14:25, 24 October 2007

Figure 1: Following two figures are selected from five parallel experiments aiming at verifying the function of immobilized amplified cells. The function curves of generator cells and block cells are also displayed in the same figure to emphasize the effects of amplified cells. Actually, the curves of amplified cell( marked as 8P) is identical with that of generator cells, a phenomenan which could be attribute to self-activation of amplified cells because of its leakage expression of AHL. Since the threshold of Quorum Sensing is far below than our expectation, further adjustment need to make to eliminate the leakage expression of AHL to a reasonal level to avoid such a condition.
TJUQ1.jpgTJUQ2.jpg



Figure 2: One of our strategy is to cut down the functional part from original plasmid and transfer into a plasmid with a copy number less than 10 without the addition of IPTG (pSB2K3). However,

TJUQ3.jpg