Tokyo/AHL assay practice
From 2007.igem.org
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<br>ΔP = promoter deleted | <br>ΔP = promoter deleted | ||
===Procedure: === | ===Procedure: === | ||
| + | <br> | ||
| + | [[Tokyo/AHL assay Standard protocol |AHL assay Standard protocol ]] | ||
<br>The final concentration of AHL is 6 pM. | <br>The final concentration of AHL is 6 pM. | ||
| + | |||
===Result & Conclusion: === | ===Result & Conclusion: === | ||
6pM of AHL is not enough to activate the AHL-activated luxR promoter. | 6pM of AHL is not enough to activate the AHL-activated luxR promoter. | ||
Revision as of 05:28, 22 October 2007
Contents |
Purpose1:
To practice AHL assay.
Purpose2:
To check if AHL works.
Samples:
luxR + GFP in DH5a (AHL-activated promoter)
placQI + GFP in DH5a (pos. con.)
pSBΔP+ GFP (neg. con.)
ΔP = promoter deleted
Procedure:
AHL assay Standard protocol
The final concentration of AHL is 6 pM.
Result & Conclusion:
6pM of AHL is not enough to activate the AHL-activated luxR promoter.
