Tokyo/AHL assay practice
From 2007.igem.org
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| + | ===Objective1: === | ||
To practice AHL assay. | To practice AHL assay. | ||
| - | === | + | ===Objective2: === |
To check if AHL works. | To check if AHL works. | ||
===Samples: === | ===Samples: === | ||
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===Result & Conclusion: === | ===Result & Conclusion: === | ||
6pM of AHL is not enough to activate the AHL-activated luxR promoter. | 6pM of AHL is not enough to activate the AHL-activated luxR promoter. | ||
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| + | == == | ||
| + | [[Tokyo/Preliminary assays |"Back to Preliminary assays"]] | ||
Latest revision as of 18:49, 26 October 2007
Objective1:
To practice AHL assay.
Objective2:
To check if AHL works.
Samples:
luxR + GFP in DH5a (AHL-activated promoter)
placQI + GFP in DH5a (pos. con.)
pSBΔP+ GFP (neg. con.)
ΔP = promoter deleted
Procedure:
AHL assay Standard protocol
The final concentration of AHL is 6 pM.
Result & Conclusion:
6pM of AHL is not enough to activate the AHL-activated luxR promoter.
