Tokyo/AHL assay practice
From 2007.igem.org
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- | === | + | __NOTOC__ |
+ | ===Objective1: === | ||
To practice AHL assay. | To practice AHL assay. | ||
- | === | + | ===Objective2: === |
To check if AHL works. | To check if AHL works. | ||
===Samples: === | ===Samples: === | ||
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<br>ΔP = promoter deleted | <br>ΔP = promoter deleted | ||
===Procedure: === | ===Procedure: === | ||
+ | <br> | ||
+ | [[Tokyo/AHL assay Standard protocol |AHL assay Standard protocol ]] | ||
<br>The final concentration of AHL is 6 pM. | <br>The final concentration of AHL is 6 pM. | ||
+ | |||
===Result & Conclusion: === | ===Result & Conclusion: === | ||
6pM of AHL is not enough to activate the AHL-activated luxR promoter. | 6pM of AHL is not enough to activate the AHL-activated luxR promoter. | ||
+ | |||
+ | == == | ||
+ | [[Tokyo/Preliminary assays |"Back to Preliminary assays"]] |
Latest revision as of 18:49, 26 October 2007
Objective1:
To practice AHL assay.
Objective2:
To check if AHL works.
Samples:
luxR + GFP in DH5a (AHL-activated promoter)
placQI + GFP in DH5a (pos. con.)
pSBΔP+ GFP (neg. con.)
ΔP = promoter deleted
Procedure:
AHL assay Standard protocol
The final concentration of AHL is 6 pM.
Result & Conclusion:
6pM of AHL is not enough to activate the AHL-activated luxR promoter.