Tokyo/AHL assay practice

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Contents

Purpose1:

To practice AHL assay.

Purpose2:

To check if AHL works.

Samples:


luxR + GFP in DH5a (AHL-activated promoter)
placQI + GFP in DH5a (pos. con.)
pSBΔP+ GFP (neg. con.)


ΔP = promoter deleted

Procedure:


The final concentration of AHL is 6 pM.

Result & Conclusion:

6pM of AHL is not enough to activate the AHL-activated luxR promoter.