Tokyo/Assay

From 2007.igem.org

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導出に関してはfor more detail.
導出に関してはfor more detail.
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= [[Tokyo/Preliminary assays |Preliminary assays ]] =
 
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[[Image:AHL Assay2.JPG|thumb|200px| '''Fig.1: '''Exogenously added AHL]]
 
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Preliminaly assays were done
 
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<br>- to practice and experience the assays with AHL and IPTG
 
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<br>- to check if and how our materials actually worked.
 
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== [[Tokyo/AHL preparation |AHL preparation]] ==
 
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== [[Tokyo/Experimental precision and accurecy|Experimental precision and accurecy]] ==
 
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== [[Tokyo/AHL assay practice|AHL assay practice]] ==
 
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== [[Tokyo/Lux-lac hybrid promoter activation check|Lux-lac hybrid promoter activation check]] ==
 
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== [[Tokyo/OD correction|OD correction]] ==
 
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== [[Tokyo/Wash|Wash]] ==
 
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== [[Tokyo/New AHL check|New AHL check]] ==
 
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== [[Tokyo/Hybrid promoter activation & repression check|Hybrid promoter activation & repression check ]] ==
 
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<br>
 
= [[Tokyo/Assays for hybrid promoter|Assays for hybrid promoter]] =
= [[Tokyo/Assays for hybrid promoter|Assays for hybrid promoter]] =

Revision as of 11:08, 22 October 2007

Hill function fitting

新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める． ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる. We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
Expression6-1.JPG
Expression6-2.JPG

導出に関してはfor more detail.

Assays for hybrid promoter

These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.


Repression and activation check with LacI and AHL

IPTG assay

Activation check by cell-produced AHL

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