Tokyo/Assay
From 2007.igem.org
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| - | = [[Tokyo/Hill function fitting |Hill function fitting ]] = | + | <br>[[Tokyo/Works|Works top]] 0.[[Tokyo/Hybrid promoter|Hybrid promoter]] 1.[[Tokyo/Formulation |Formulation]] 2.[[Tokyo/Assay |Assay1]] 3.[[Tokyo/Simulation |Simulation]] 4.[[Tokyo/Assay2 |Assay2]] 5.[[Tokyo/Future works |Future works]] |
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| + | =1.Important assay = | ||
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| + | ==[[Tokyo/Hill function fitting |Hill function fitting ]] == | ||
新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める． | 新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める． | ||
ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる. | ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる. | ||
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導出に関してはfor more detail. | 導出に関してはfor more detail. | ||
| - | + | == [[Tokyo/Assays for hybrid promoter|Assays for hybrid promoter]] == | |
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| - | = [[Tokyo/Assays for hybrid promoter|Assays for hybrid promoter]] = | + | |
These assays were done | These assays were done | ||
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== [[Tokyo/Repression and activation check with LacI and AHL|Repression and activation check with LacI and AHL]] == | == [[Tokyo/Repression and activation check with LacI and AHL|Repression and activation check with LacI and AHL]] == | ||
| + | 目的 | ||
| + | 結果 | ||
== [[Tokyo/IPTG assay|IPTG assay]] == | == [[Tokyo/IPTG assay|IPTG assay]] == | ||
| + | 目的 | ||
| + | 結果 | ||
== [[Tokyo/Activation check by cell-produced AHL |Activation check by cell-produced AHL ]] == | == [[Tokyo/Activation check by cell-produced AHL |Activation check by cell-produced AHL ]] == | ||
| + | 目的 | ||
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| + | 結果 | ||
| + | =2.Preliminary assays= | ||
| + | '''We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.''' | ||
| + | <br>[[Tokyo/Preliminary assays |See more details]] | ||
Latest revision as of 14:06, 23 October 2007
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
1.Important assay
Hill function fitting
新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める．
ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる.
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
導出に関してはfor more detail.
Assays for hybrid promoter
These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.
Repression and activation check with LacI and AHL
目的
結果
IPTG assay
目的
結果
Activation check by cell-produced AHL
目的
結果
2.Preliminary assays
We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.
See more details
