Tokyo/Assay

From 2007.igem.org

Revision as of 11:09, 22 October 2007 by Nakatani (Talk | contribs)

Hill function fitting

新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める. ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる. We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
Expression6-1.JPG
Expression6-2.JPG

導出に関してはfor more detail.

Assays for hybrid promoter

These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.


Repression and activation check with LacI and AHL

IPTG assay

Activation check by cell-produced AHL

Preliminary assays