Tokyo/Formulation/2.toggle model with hybrid promoter

From 2007.igem.org

(Difference between revisions)
Line 1: Line 1:
-
 
<br>[[Image:AHLresponse2-1.jpg|300px|]]
<br>[[Image:AHLresponse2-1.jpg|300px|]]
-
<br>AHLを産生しない系を仮定し,外から入れたAHL量に対してどのような振る舞いを示すか見てみる.
+
<br>Assuming the system where ''E. coli'' does not produce AHL by themselves, we have observed the system's reaction to ''ab extra'' AHL. The ODEs were obtained as
-
<br>定式化において,プロモータbはAHLによってactivateされる項を加える必要がある.すると,以下のようになる.
+
-
<br>Assuming the system where ''E. coli'' does not produce AHL by themselves, we have observed the system's reaction to ''ab extra'' AHL.
 
-
<br>In formulation, it is necessary to add the term of promoter b activation by AHL as follows;
 
<br>[[Image:expression2-1.jpg|200px|]]
<br>[[Image:expression2-1.jpg|200px|]]
-
<br>我々のモデルの理想的な振る舞いは図のようである.
+
<br>where the factor surrounded in the orange box represents the activation of promoter B.
-
<br>AHLが少ないとき,B状態の大腸菌は不安定化してA状態に遷移する.一方,AHLが多いときは,A状態の大腸菌,B状態の大腸菌はそれぞれ安定化している.
+
 
<br>The ideal behavior shown in the figure below is:
<br>The ideal behavior shown in the figure below is:
-
<br>- with little AHL, the B state becomes unstable and shifts to A.
+
<br>- with low concentration of AHL, the B state becomes unstable. so that the cells at the B state move to the A state which is stable.
-
<br>- with lots of AHL, both A and B states are stable.
+
<br>- with hign concentration of AHL, both A and B states are stable.The cells at the A state keep the A state,that at the B state keep the B state. 
<br>[[Image:AHLresponse2-2.jpg|300px|]]
<br>[[Image:AHLresponse2-2.jpg|300px|]]

Revision as of 12:52, 23 October 2007


AHLresponse2-1.jpg


Assuming the system where E. coli does not produce AHL by themselves, we have observed the system's reaction to ab extra AHL. The ODEs were obtained as


Expression2-1.jpg


where the factor surrounded in the orange box represents the activation of promoter B.


The ideal behavior shown in the figure below is:
- with low concentration of AHL, the B state becomes unstable. so that the cells at the B state move to the A state which is stable.
- with hign concentration of AHL, both A and B states are stable.The cells at the A state keep the A state,that at the B state keep the B state. 


AHLresponse2-2.jpg


実際に,式を解いて相平面を書いてみると図のようになる.
The phaseplane drawn by solving the equations is the following:


Expression2-2.jpg



AHLresponse2-3.jpgAHLresponse2-4.jpgAHLresponse2-5.jpg