Tokyo/Wash

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(Wash)
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== Wash ==
== Wash ==
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[[Image:wash.jpg]]
<br>take 200 ml of culture in a Falcon tube into a eppendorf tube (1.6 or 2.0 ml tube)
<br>take 200 ml of culture in a Falcon tube into a eppendorf tube (1.6 or 2.0 ml tube)
<br>centrifugation for 2 min at 9000g
<br>centrifugation for 2 min at 9000g

Revision as of 09:08, 20 October 2007

Wash

Wash.jpg
take 200 ml of culture in a Falcon tube into a eppendorf tube (1.6 or 2.0 ml tube)
centrifugation for 2 min at 9000g
discard the supernatant with a pippet
add 200 ml of PBS and dissolve the pellet
centrifugation for 2 min at 9000g
discard the supernatant with a pippet
add 200 ml of PBS and dissolve the pellet
mix well
apply 150 ml onto a well