Tokyo/Wash
From 2007.igem.org
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<br>apply 150 ml onto a well | <br>apply 150 ml onto a well | ||
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| + | [[Tokyo/Preliminary assays |"Back to Preliminary assays"]] | ||
Latest revision as of 07:09, 25 October 2007
Wash
take 200 ml of culture in a Falcon tube into a eppendorf tube (1.6 or 2.0 ml tube)
centrifugation for 2 min at 9000g
discard the supernatant with a pippet
add 200 ml of PBS and dissolve the pellet
centrifugation for 2 min at 9000g
discard the supernatant with a pippet
add 200 ml of PBS and dissolve the pellet
mix well
apply 150 ml onto a well
