http://2007.igem.org/wiki/index.php?title=Tokyo/Works/Assay0&feed=atom&action=history
Tokyo/Works/Assay0 - Revision history
2024-03-28T09:06:22Z
Revision history for this page on the wiki
MediaWiki 1.16.5
http://2007.igem.org/wiki/index.php?title=Tokyo/Works/Assay0&diff=44282&oldid=prev
Iwasaki: /* Samples: */
2007-10-26T19:57:31Z
<p><span class="autocomment">Samples:</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:57, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 26:</td>
<td colspan="2" class="diff-lineno">Line 26:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>[Lux lac hybrid promoter – GFP] is A-4' + lux lac hybrid promoter, for example. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>[Lux lac hybrid promoter – GFP] is A-4' + lux lac hybrid promoter, for example. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>'''Repressor LacI expression:''' </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>'''Repressor LacI expression:''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>pTrc99A expresses LacI</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>pTrc99A expresses LacI</div></td></tr>
</table>
Iwasaki
http://2007.igem.org/wiki/index.php?title=Tokyo/Works/Assay0&diff=44253&oldid=prev
Iwasaki: /* Samples: */
2007-10-26T19:55:02Z
<p><span class="autocomment">Samples:</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:55, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 29:</td>
<td colspan="2" class="diff-lineno">Line 29:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>'''Repressor LacI expression:''' </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>'''Repressor LacI expression:''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>pTrc99A expresses LacI</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>pTrc99A expresses LacI</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>pBR322 TetR does NOT express LacI</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>pBR322 TetR does NOT express LacI <ins class="diffchange diffchange-inline">(but expresses TetR, so the condition is more similar than "empty" DH5alpha)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>'''Antibiotics resistance:''' </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>'''Antibiotics resistance:''' </div></td></tr>
</table>
Iwasaki
http://2007.igem.org/wiki/index.php?title=Tokyo/Works/Assay0&diff=44228&oldid=prev
Nakatani: /* Results and Conclusion */
2007-10-26T19:53:48Z
<p><span class="autocomment">Results and Conclusion</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:53, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 44:</td>
<td colspan="2" class="diff-lineno">Line 44:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:hybridgraph2.JPG|thumb|350px| '''Fig.1: '''Result. Activities of the newly devised lux lac hybrid promoter (hy) were determined in the presence and absence of LacI (lacI(+) and lacI(-), respectively) and those of AHL (AHL(+) and AHL(-)).]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:hybridgraph2.JPG|thumb|350px| '''Fig.1: '''Result. Activities of the newly devised lux lac hybrid promoter (hy) were determined in the presence and absence of LacI (lacI(+) and lacI(-), respectively) and those of AHL (AHL(+) and AHL(-)).]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:<del class="diffchange diffchange-inline">hybridgraph</del>.JPG|thumb|300px| '''Fig.2: '''Result summary]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:<ins class="diffchange diffchange-inline">hybridgraph3</ins>.JPG|thumb|300px| '''Fig.2: '''Result summary]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''The newly devised hybrid promoter is activated by AHL and repressed by LacI.''' </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''The newly devised hybrid promoter is activated by AHL and repressed by LacI.''' </div></td></tr>
</table>
Nakatani
http://2007.igem.org/wiki/index.php?title=Tokyo/Works/Assay0&diff=44187&oldid=prev
Iwasaki: /* Samples: */
2007-10-26T19:50:43Z
<p><span class="autocomment">Samples:</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:50, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 34:</td>
<td colspan="2" class="diff-lineno">Line 34:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>pTrc99A gives ampicillin-resistance</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>pTrc99A gives ampicillin-resistance</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>pBR322 TetR gives ampicillin-resistance</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>pBR322 TetR gives ampicillin-resistance</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>[LacI hybrid promoter – GFP] gives kanamicin-resistance</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>[LacI hybrid promoter – GFP] <ins class="diffchange diffchange-inline"> ([http://partsregistry.org/Part:BBa_I751900 BBa_I751900]) </ins>gives kanamicin-resistance</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>[<del class="diffchange diffchange-inline">placQI </del>– GFP] gives kanamicin-resistance</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>[<ins class="diffchange diffchange-inline">placIQ </ins>– GFP] <ins class="diffchange diffchange-inline">([http://partsregistry.org/Part:BBa_J54201 BBa_J54201]) </ins>gives kanamicin-resistance</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Procedure:===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Procedure:===</div></td></tr>
</table>
Iwasaki
http://2007.igem.org/wiki/index.php?title=Tokyo/Works/Assay0&diff=44169&oldid=prev
Iwasaki: /* Samples: */
2007-10-26T19:48:44Z
<p><span class="autocomment">Samples:</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:48, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 22:</td>
<td colspan="2" class="diff-lineno">Line 22:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> --></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> --></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><!--</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>*Promoters are inserted into the BioBrick part A-4 with its promoter pluxR deleted (A-4').</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>*Promoters are inserted into the BioBrick part A-4 with its promoter pluxR deleted (A-4').</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>[Lux lac hybrid promoter – GFP] is A-4' + lux lac hybrid promoter, for example. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>[Lux lac hybrid promoter – GFP] is A-4' + lux lac hybrid promoter, for example. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">--></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>'''Repressor LacI expression:''' </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>'''Repressor LacI expression:''' </div></td></tr>
</table>
Iwasaki
http://2007.igem.org/wiki/index.php?title=Tokyo/Works/Assay0&diff=44135&oldid=prev
Iwasaki: /* Samples: */
2007-10-26T19:45:55Z
<p><span class="autocomment">Samples:</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:45, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 18:</td>
<td colspan="2" class="diff-lineno">Line 18:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>4-2. pTrc99A + [A4 ΔP] ([http://partsregistry.org/Part:BBa_I751100 BBa_I751100])<!--[LacI promoter – GFP] -->(Neg. con.) (AHL-)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>4-2. pTrc99A + [A4 ΔP] ([http://partsregistry.org/Part:BBa_I751100 BBa_I751100])<!--[LacI promoter – GFP] -->(Neg. con.) (AHL-)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><!-- <br>5-1. pBR322 TetR +<del class="diffchange diffchange-inline"><!-- </del>[LacI promoter – GFP] <del class="diffchange diffchange-inline">--></del>(Neg. con. check) (AHL+)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><!-- <br>5-1. pBR322 TetR + [LacI promoter – GFP] (Neg. con. check) (AHL+)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>5-2. pBR322 TetR + <del class="diffchange diffchange-inline"><!--</del>[LacI promoter – GFP] <del class="diffchange diffchange-inline">--></del>(Neg. con. check) (AHL-)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>5-2. pBR322 TetR + [LacI promoter – GFP] (Neg. con. check) (AHL-)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>--></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>--></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>
Iwasaki
http://2007.igem.org/wiki/index.php?title=Tokyo/Works/Assay0&diff=44118&oldid=prev
Iwasaki: /* Samples: */
2007-10-26T19:43:29Z
<p><span class="autocomment">Samples:</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:43, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 14:</td>
<td colspan="2" class="diff-lineno">Line 14:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>2-2. pBR322 TetR + [Lux lac hybrid promoter – GFP] ([http://partsregistry.org/Part:BBa_I751900 BBa_I751900]) '''(lacI-) (AHL-)'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>2-2. pBR322 TetR + [Lux lac hybrid promoter – GFP] ([http://partsregistry.org/Part:BBa_I751900 BBa_I751900]) '''(lacI-) (AHL-)'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>3-1. pTrc99A + [placIQ(constitutive) – GFP] ([http://partsregistry.org/Part:BBa_J54201 BBa_J54201]) (Pos. con.) (AHL+)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>3-1. pTrc99A + [placIQ(constitutive) – GFP] ([http://partsregistry.org/Part:BBa_J54201 BBa_J54201]) (Pos. con.) (AHL+)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>3-2. pTrc99A + [placIQ(constitutive) – GFP] (http://partsregistry.org/Part:BBa_J54201 BBa_J54201) (Pos. con.) (AHL-)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>3-2. pTrc99A + [placIQ(constitutive) – GFP] (<ins class="diffchange diffchange-inline">[</ins>http://partsregistry.org/Part:BBa_J54201 BBa_J54201<ins class="diffchange diffchange-inline">]</ins>) (Pos. con.) (AHL-)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>4-1. pTrc99A + [LacI promoter – GFP] (Neg. con.) (AHL+)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>4-1. pTrc99A + <ins class="diffchange diffchange-inline">[A4 ΔP] ([http://partsregistry.org/Part:BBa_I751100 BBa_I751100])<!-- </ins>[LacI promoter – GFP] <ins class="diffchange diffchange-inline">--> </ins>(Neg. con.) (AHL+)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>4-2. pTrc99A + [LacI promoter – GFP] (Neg. con.) (AHL-)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>4-2. pTrc99A + <ins class="diffchange diffchange-inline">[A4 ΔP] ([http://partsregistry.org/Part:BBa_I751100 BBa_I751100])<!--</ins>[LacI promoter – GFP] <ins class="diffchange diffchange-inline"> --></ins>(Neg. con.) (AHL-)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>5-1. pBR322 TetR + [LacI promoter – GFP] (Neg. con. check) (AHL+)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>5-2. pBR322 TetR + [LacI promoter – GFP] (Neg. con. check) (AHL-)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><!-- </ins><br>5-1. pBR322 TetR +<ins class="diffchange diffchange-inline"><!-- </ins>[LacI promoter – GFP] <ins class="diffchange diffchange-inline"> --></ins>(Neg. con. check) (AHL+)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>5-2. pBR322 TetR + <ins class="diffchange diffchange-inline"><!--</ins>[LacI promoter – GFP] <ins class="diffchange diffchange-inline"> --></ins>(Neg. con. check) (AHL-)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">--></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>*Promoters are inserted into the BioBrick part A-4 with its promoter pluxR deleted (A-4').</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>*Promoters are inserted into the BioBrick part A-4 with its promoter pluxR deleted (A-4').</div></td></tr>
</table>
Iwasaki
http://2007.igem.org/wiki/index.php?title=Tokyo/Works/Assay0&diff=44013&oldid=prev
Iwasaki: /* Samples: */
2007-10-26T19:31:09Z
<p><span class="autocomment">Samples:</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:31, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 13:</td>
<td colspan="2" class="diff-lineno">Line 13:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>2-1. pBR322 TetR + [Lux lac hybrid promoter – GFP] ([http://partsregistry.org/Part:BBa_I751900 BBa_I751900]) '''(lacI-) (AHL+)'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>2-1. pBR322 TetR + [Lux lac hybrid promoter – GFP] ([http://partsregistry.org/Part:BBa_I751900 BBa_I751900]) '''(lacI-) (AHL+)'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>2-2. pBR322 TetR + [Lux lac hybrid promoter – GFP] ([http://partsregistry.org/Part:BBa_I751900 BBa_I751900]) '''(lacI-) (AHL-)'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>2-2. pBR322 TetR + [Lux lac hybrid promoter – GFP] ([http://partsregistry.org/Part:BBa_I751900 BBa_I751900]) '''(lacI-) (AHL-)'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>3-1. pTrc99A + [<del class="diffchange diffchange-inline">placQI </del>– GFP] (<del class="diffchange diffchange-inline">placQI is constitutive promoter</del>) (Pos. con.) (AHL+)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>3-1. pTrc99A + [<ins class="diffchange diffchange-inline">placIQ(constitutive) </ins>– GFP] (<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_J54201 BBa_J54201]</ins>) (Pos. con.) (AHL+)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>3-2. pTrc99A + [<del class="diffchange diffchange-inline">placQI </del>– GFP] (<del class="diffchange diffchange-inline">placQI is constitutive promoter</del>) (Pos. con.) (AHL-)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>3-2. pTrc99A + [<ins class="diffchange diffchange-inline">placIQ(constitutive) </ins>– GFP] (<ins class="diffchange diffchange-inline">http://partsregistry.org/Part:BBa_J54201 BBa_J54201</ins>) (Pos. con.) (AHL-)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>4-1. pTrc99A + [LacI promoter – GFP] (Neg. con.) (AHL+)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>4-1. pTrc99A + [LacI promoter – GFP] (Neg. con.) (AHL+)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>4-2. pTrc99A + [LacI promoter – GFP] (Neg. con.) (AHL-)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>4-2. pTrc99A + [LacI promoter – GFP] (Neg. con.) (AHL-)</div></td></tr>
</table>
Iwasaki
http://2007.igem.org/wiki/index.php?title=Tokyo/Works/Assay0&diff=43999&oldid=prev
Iwasaki: /* Samples: */
2007-10-26T19:29:35Z
<p><span class="autocomment">Samples:</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:29, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 9:</td>
<td colspan="2" class="diff-lineno">Line 9:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Samples: ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Samples: ===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>1-1. pTrc99A + [Lux lac hybrid promoter – GFP] <del class="diffchange diffchange-inline"> </del>'''(LacI+) (AHL+)'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>1-1. pTrc99A + [Lux lac hybrid promoter – GFP] <ins class="diffchange diffchange-inline">([http://partsregistry.org/Part:BBa_I751900 BBa_I751900]) </ins>'''(LacI+) (AHL+)'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>1-2. pTrc99A + [Lux lac hybrid promoter – GFP] '''(LacI+) (AHL-)'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>1-2. pTrc99A + [Lux lac hybrid promoter – GFP] <ins class="diffchange diffchange-inline">([http://partsregistry.org/Part:BBa_I751900 BBa_I751900]) </ins> '''(LacI+) (AHL-)'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>2-1. pBR322 TetR + [Lux lac hybrid promoter – GFP] '''(lacI-) (AHL+)'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>2-1. pBR322 TetR + [Lux lac hybrid promoter – GFP] <ins class="diffchange diffchange-inline"> ([http://partsregistry.org/Part:BBa_I751900 BBa_I751900]) </ins>'''(lacI-) (AHL+)'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>2-2. pBR322 TetR + [Lux lac hybrid promoter – GFP] '''(lacI-) (AHL-)'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>2-2. pBR322 TetR + [Lux lac hybrid promoter – GFP] <ins class="diffchange diffchange-inline"> ([http://partsregistry.org/Part:BBa_I751900 BBa_I751900]) </ins>'''(lacI-) (AHL-)'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>3-1. pTrc99A + [placQI – GFP] (placQI is constitutive promoter) (Pos. con.) (AHL+)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>3-1. pTrc99A + [placQI – GFP] (placQI is constitutive promoter) (Pos. con.) (AHL+)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>3-2. pTrc99A + [placQI – GFP] (placQI is constitutive promoter) (Pos. con.) (AHL-)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>3-2. pTrc99A + [placQI – GFP] (placQI is constitutive promoter) (Pos. con.) (AHL-)</div></td></tr>
</table>
Iwasaki
http://2007.igem.org/wiki/index.php?title=Tokyo/Works/Assay0&diff=40683&oldid=prev
Hiro: /* Procedure: */
2007-10-26T10:57:07Z
<p><span class="autocomment">Procedure:</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 10:57, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 35:</td>
<td colspan="2" class="diff-lineno">Line 35:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Procedure:===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Procedure:===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>[[Tokyo/AHL assay Standard protocol |AHL assay Standard protocol ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>[[Tokyo/AHL assay Standard protocol |AHL assay Standard protocol ]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>The <del class="diffchange diffchange-inline">concentration </del>of AHL and IPTG, if added, were 10 nM and 1 mM, respectively.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>The <ins class="diffchange diffchange-inline">concentrations </ins>of AHL and IPTG, if added, were 10 nM and 1 mM, respectively.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Results and Conclusion===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Results and Conclusion===</div></td></tr>
</table>
Hiro