Tokyo/suggestions

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Suggestion 1.
Fig. 1 Suggestion 1
Merit:
The repressor R1 can be inserted after constructing the other part.


Suggestion 2.
Fig. 2 Suggestion 2
Merit:
A ribosome binding site here is easily exchangeable; therefore, the expression level can be modulated without changing the promoter and regulators.


Suggestion 3.
Fig. 3 Suggestion 3
Merit:
Promoters can be easily exchanged, and the expression level can be modulated at promoter level.


Suggestion 4.
Fig. 4 Suggestion 4
Merit:
The precursor of this part was constructed for the functional check of BioBrick parts and available;
therefore, this construction can be done in much shorter time.


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