From 2007.igem.org

Growing colonies in broth:

1. Prepare 5mL LB + Amp broth in a sterile Falcon tube. 2. Pick up a colony from the plate by a micropipette tip or sterile toothpick and put it in the falcon. 3. Incubate at 37C incubator for 14-16 hours.

Miniprep Plasmid Isolation (with Qiagen kit):

1. Centrifuge the falcons at 4000rpm for 4-10 minutes. 2.Resuspend pelleted bacterial cells in 250ML Buffer P1(kept in +4C) and transfer to a 1.5mL eppendorf. 3.Add 250ML Buffer P2 and invert the tube for 6 times to mix (do not vortex).