UCSF

Location, Location, Location:
Directing Biology through Synthetic Assemblies and Organelles

Project 1: Protein Scaffolds as a Molecular Breadboard

Using synthetic protein scaffolds to control information flow of a kinase pathway in eukaryotic cells.

Project 2: Creating a Synthetic Organelle

Engineering phosphoinositide "barcodes" to create an intracellular membrane-bound chassis for

synthetic biology applications in eukaryotic cells.

People

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Students

• Michael Chen (UC Berkeley)
• Eric Chou (Lincoln High)
• Jimmy Huang (Lincoln High)
• Lauren Jann (Lincoln High)
• Eric Meltzer (Palo Alto High)
• Alexander Ng (Lincoln High)
• Robert Ovadia (Lincoln High)

Instructors

• Noah Helman
• Andrew Horwitz
• Sergio Peisajovich
• David Pincus
• Ryan Ritterson
• Nili Sommovilla
• Reid Williams
• Angela Won

Faculty Advisors

• [http://www.ucsf.edu/limlab/ Wendell Lim]
• Hana El-Samad
• [http://kortemmelab.ucsf.edu/ Tanja Kortemme]
• [http://www.voigtlab.ucsf.edu/ Chris Voigt]

The Project

About Us

• Our Story

A Guided Tour through the Project: JUDGES, THIS IS FOR YOU!

• Project Description
• Main Introduction
• Project One: Engineering Protein Complexes
  • Intro
  • Results
  • Conclusions
• Project Two: The Synthetic Organelle
  • Intro
  • Results
  • Status
• Overall Conclusions

Behind the Scenes

• Methods and Materials
  • Project One
    • Cloning Strategy
    • Yeast Strains
    • Flow Cytometry
  • Project Two
    • Cloning Strategy
    • Yeast Strains
    • Flow Cytometry
    • Microscopy
• Plasmids & Sequences

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